RRC ID 52878
Author Kohzaki H.
Title The function of replication and SCF complex during Drosophila wing development.
Journal Front Biosci (Landmark Ed)
Abstract Chromosomal DNA replication machinery functions in the growing cells and organs in multicellular organisms. We previously demonstrated that its knockdown in several tissues of Drosophila led to a rough eye phenotype, the loss of bristles in the eye and female sterile. In this paper, I investigated in detail the wing phenotype using RNAi flies, and observed that the knockdown not only of Mcm10 but also of some other prereplicative complex components including Cdt1, Polα-primase, RPA, Psf2 (partner of SLD five 2; a subunit of GINS (Go, Ichi, Nii, and San; five, one, two, and three in Japanese) and Rfc3 (replication factor C 3; a subunit of RFC complex) demonstrated wing phenotypes, using Gal4-driver flies. Surprisingly, some SCF complex components, which control cell cycle progression via protein degradation, also showed the wing phenotype. These results showed that the DNA replication machinery contributes to wing development independently of growth, probably through defects in DNA replication and protein degradation at specific places and times.
Volume 23(12)
Pages 2235-2244
Published 2018-6-1
DOI 10.2741/4702
PII 4702
PMID 29772558
MeSH Animals Animals, Genetically Modified Cell Cycle / genetics DNA Replication* Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila melanogaster / genetics* Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism Gene Expression Regulation, Developmental* Microscopy, Electron, Scanning Minichromosome Maintenance Proteins / genetics Minichromosome Maintenance Proteins / metabolism Multiprotein Complexes / genetics* Multiprotein Complexes / metabolism RNA Interference Wings, Animal / growth & development Wings, Animal / metabolism* Wings, Animal / ultrastructure
IF 2.747
Times Cited 1
Resource
Drosophila 1616R-1 1616R-3 4082R-1 4082R-2 5971R-3 8171R-2 8171R-3  2917R-2 2917R-3  7833R-1 9633R-1 9633R-3 9187R-3 18013R-1 5313R-9 6349R-1 6349R-3 7108-1R-1 7108-2R-1  5949R-1 6768R-1  6768R-2 5170R-1