RRC ID 52884
Author Schrick K, Bruno M, Khosla A, Cox PN, Marlatt SA, Roque RA, Nguyen HC, He C, Snyder MP, Singh D, Yadav G.
Title Shared functions of plant and mammalian StAR-related lipid transfer (START) domains in modulating transcription factor activity.
Journal BMC Biol.
Abstract BACKGROUND:Steroidogenic acute regulatory protein (StAR)-related lipid transfer (START) domains were first identified from mammalian proteins that bind lipid/sterol ligands via a hydrophobic pocket. In plants, predicted START domains are predominantly found in homeodomain leucine zipper (HD-Zip) transcription factors that are master regulators of cell-type differentiation in development. Here we utilized studies of Arabidopsis in parallel with heterologous expression of START domains in yeast to investigate the hypothesis that START domains are versatile ligand-binding motifs that can modulate transcription factor activity.
RESULTS:Our results show that deletion of the START domain from Arabidopsis Glabra2 (GL2), a representative HD-Zip transcription factor involved in differentiation of the epidermis, results in a complete loss-of-function phenotype, although the protein is correctly localized to the nucleus. Despite low sequence similarly, the mammalian START domain from StAR can functionally replace the HD-Zip-derived START domain. Embedding the START domain within a synthetic transcription factor in yeast, we found that several mammalian START domains from StAR, MLN64 and PCTP stimulated transcription factor activity, as did START domains from two Arabidopsis HD-Zip transcription factors. Mutation of ligand-binding residues within StAR START reduced this activity, consistent with the yeast assay monitoring ligand-binding. The D182L missense mutation in StAR START was shown to affect GL2 transcription factor activity in maintenance of the leaf trichome cell fate. Analysis of in vivo protein-metabolite interactions by mass spectrometry provided direct evidence for analogous lipid-binding activity in mammalian and plant START domains in the yeast system. Structural modeling predicted similar sized ligand-binding cavities of a subset of plant START domains in comparison to mammalian counterparts.
CONCLUSIONS:The START domain is required for transcription factor activity in HD-Zip proteins from plants, although it is not strictly necessary for the protein's nuclear localization. START domains from both mammals and plants are modular in that they can bind lipid ligands to regulate transcription factor function in a yeast system. The data provide evidence for an evolutionarily conserved mechanism by which lipid metabolites can orchestrate transcription. We propose a model in which the START domain is used by both plants and mammals to regulate transcription factor activity.
Volume 12
Pages 70
Published 2014-8-27
DOI 10.1186/s12915-014-0070-8
PII s12915-014-0070-8
PMID 25159688
PMC PMC4169639
MeSH Animals Arabidopsis / genetics* Arabidopsis / metabolism Arabidopsis Proteins / chemistry Arabidopsis Proteins / genetics* Arabidopsis Proteins / metabolism Gene Expression Regulation, Plant* Homeodomain Proteins / chemistry Homeodomain Proteins / genetics* Homeodomain Proteins / metabolism Ligands Mass Spectrometry Mice Organisms, Genetically Modified / genetics Phosphoproteins / chemistry Phosphoproteins / genetics* Phosphoproteins / metabolism Protein Structure, Tertiary Saccharomyces cerevisiae / genetics Transcription Factors / chemistry Transcription Factors / genetics Transcription Factors / metabolism*
IF 6.723
Times Cited 28
Resource
Arabidopsis / Cultured plant cells, genes pda01653 pda03562 pda12740 pda10896 pda04064 pda06690 pda06113 pda08603 pda06038 pda09256 pda01557 pda09731 pda07478 pda10040 pda12825 pda05716