| RRC ID |
52894
|
| 著者 |
Verma A, Pal Y, Khatri I, Ojha AK, Gruber-Vodicka H, Schumann P, Dastager S, Subramanian S, Mayilraj S, Krishnamurthi S.
|
| タイトル |
Examination into the taxonomic position of Bacillus thermotolerans Yang et al., 2013, proposal for its reclassification into a new genus and species Quasibacillus thermotolerans gen. nov., comb. nov. and reclassification of B. encimensis Dastager et al., 2015 as a later heterotypic synonym of B. badius.
|
| ジャーナル |
Syst Appl Microbiol
|
| Abstract |
Two novel Gram-staining positive, rod-shaped, moderately halotolerant, endospore forming bacterial strains 5.5LF 38TD and 5.5LF 48TD were isolated and taxonomically characterized from a landfill in Chandigarh, India. The analysis of 16S rRNA gene sequences of the strains confirmed their closest identity to Bacillus thermotolerans SgZ-8T with 99.9% sequence similarity. A comparative phylogenetic analysis of strains 5.5LF 38TD, 5.5LF 48TD and B. thermotolerans SgZ-8T confirmed their separation into a novel genus with B. badius and genus Domibacillus as the closest phylogenetic relatives. The major fatty acids of the strains are iso-C15:0 and iso-C16:0 and MK-7 is the only quinone. The major polar lipids are diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The digital DNA-DNA hybridization (DDH) and ortho average nucleotide identity (ANI) values calculated through whole genome sequences indicated that the three strains showed low relatedness with their phylogenetic neighbours. Based on evidences from phylogenomic analyses and polyphasic taxonomic characterization we propose reclassification of the species B. thermotolerans into a novel genus named Quasibacillus thermotolerans gen. nov., comb. nov with the type strain SgZ-8T (=CCTCC AB2012108T=KACC 16706T). Further our analyses also revealed that B. encimensis SGD-V-25T is a later heterotypic synonym of Bacillus badius DSM 23T.
|
| 巻・号 |
40(7)
|
| ページ |
411-422
|
| 公開日 |
2017-10-1
|
| DOI |
10.1016/j.syapm.2017.07.010
|
| PII |
S0723-2020(17)30098-X
|
| PMID |
28947104
|
| MeSH |
Bacillus / classification*
Bacillus / genetics*
Bacterial Typing Techniques
DNA, Bacterial / genetics
Fatty Acids / analysis
Nucleic Acid Hybridization
Phylogeny
RNA, Ribosomal, 16S / genetics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
|
| IF |
3.224
|
| 引用数 |
2
|
| リソース情報 |
| 一般微生物 |
JCM 17333
JCM 17334 |
