| RRC ID |
52931
|
| 著者 |
Wang C, Wang Z, Luo F, Li Y.
|
| タイトル |
The augmented lipid productivity in an emerging oleaginous model alga Coccomyxa subellipsoidea by nitrogen manipulation strategy.
|
| ジャーナル |
World J Microbiol Biotechnol
|
| Abstract |
The lipid productivity controlled by both of biomass and lipid content was really crucial for economic-feasibility of microalgae-based biofuels production. This study attempted at augmenting lipid productivity in an emerging oleaginous model alga Coccomyxa subellipsoidea by different nitrogen manipulation including one-stage continuous N-sufficiency (OCNS), N-deprivation (OCND), N-limitation (OCNL), and also two-stage batch N-starvation (TBNS). Amongst four tested nitrogen manipulation strategies, OCNS performed remarkable promoting effect on cell metabolic growth and the maximum biomass was achieved by 7.39 g/L. Whereas TBNS regime induced the highest lipid content (over 50.5%). Only OCNL treatment augmented the lipid productivity by 232.37 mg/L/day, representing 1.25-fold more than TBNS and even as much as 5.06-fold more than that of OCND strategy. OCNL also strengthened the proportions of saturated (C16:0 and C18:0) and monounsaturated fatty acid (C18:1) which were inclined to high-quality biofuels-making. This might be due to that most part of energy and metabolic flux (e.g. acetyl-CoA) derived from TCA cycle and glycolysis flowed into fatty acids biosynthesis pathway (especially C18:1) response to OCNL manipulation. This study represented a pioneering work of utilizing OCNL for lipids production by C. subellipsoidea and clearly implied that OCNL might be a feasible way for algal lipid production on a commercial scale and also promoted the potential of C. subellipsoidea as an ideal biodiesel feedstock.
|
| 巻・号 |
33(8)
|
| ページ |
160
|
| 公開日 |
2017-8-1
|
| DOI |
10.1007/s11274-017-2324-4
|
| PII |
10.1007/s11274-017-2324-4
|
| PMID |
28752265
|
| MeSH |
Biofuels
Biomass
Chlorophyta / growth & development
Chlorophyta / metabolism*
Culture Media
Fatty Acids / biosynthesis
Lipids / biosynthesis*
Metabolic Flux Analysis
Microalgae / growth & development
Microalgae / metabolism
Nitrogen / metabolism*
|
| IF |
2.1
|
| 引用数 |
8
|
| リソース情報 |
| 藻類 |
NIES-2166 |
