RRC ID 5301
著者 Aizawa T, Ve NB, Kimoto KI, Iwabuchi N, Sumida H, Hasegawa I, Sasaki S, Tamura T, Kudo T, Suzuki KI, Nakajima M, Sunairi M.
タイトル Curtobacterium ammoniigenes sp. nov., an ammonia-producing bacterium isolated from plants inhabiting acidic swamps in actual acid sulfate soil areas of Vietnam.
ジャーナル Int J Syst Evol Microbiol
Abstract The ammonia-producing bacteria B55(T), CA73, SA69 and SA72 were isolated from the waterweeds Ludwigia adscendens (B55(T)) and Eleocharis dulcis (CA73, SA69 and SA72) grown in highly acidic swamps (pH 2-4) in actual acid sulfate soil areas of Vietnam. The isolates were Gram-positive, irregular rod-shaped, non-spore-forming bacteria. On the basis of 16S rRNA gene sequence similarity, strain B55(T) was shown to belong to the genus Curtobacterium of the class Actinobacteria. Chemotaxonomic data (MK-9 as major isoprenoid quinone, d-ornithine as cell-wall diamino acid, acetyl as the acyl type of peptidoglycan) supported the affiliation of all four strains to this genus. Although their 16S rRNA gene sequence similarity was 99 % to species with validly published names within the genus, they formed a group that was distinct in the phylogenetic tree, and DNA-DNA relatedness values to these established species were less than 10 %. The results of physiological and biochemical tests and major fatty acids (cyclohexyl-C(17 : 0), anteiso-C(17 : 0) and cyclohexyl-C(19 : 0)) allowed phenotypic differentiation of these strains from the species of Curtobacterium with validly published names. Therefore, strains B55(T), CA73, SA69 and SA72 represent a novel species, for which the name Curtobacterium ammoniigenes sp. nov. is proposed. The type strain is B55(T) (=NBRC 101786(T)=VTCC D6-11(T)=JCM 14609(T)).
巻・号 57(Pt 7)
ページ 1447-1452
公開日 2007-7-1
DOI 10.1099/ijs.0.64731-0
PMID 17625173
MeSH Actinomycetales / chemistry Actinomycetales / classification* Actinomycetales / genetics Actinomycetales / isolation & purification* Ammonia / metabolism* Bacterial Typing Techniques Cell Wall / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Eleocharis / microbiology* Fatty Acids / analysis Genes, rRNA Molecular Sequence Data Nucleic Acid Hybridization Onagraceae / microbiology* Ornithine / analysis Peptidoglycan / analysis Phylogeny Quinones / analysis RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sequence Homology, Nucleic Acid Spores, Bacterial Vietnam Wetlands
IF 2.415
引用数 20
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM1480 JCM1350 JCM1345 JCM9670 JCM14609 JCM14610 JCM14611 JCM14612