論文 - 詳細
| RRC ID | 5304 |
|---|---|
| 著者 | Pikuta EV, Marsic D, Itoh T, Bej AK, Tang J, Whitman WB, Ng JD, Garriott OK, Hoover RB. |
| タイトル | Thermococcus thioreducens sp. nov., a novel hyperthermophilic, obligately sulfur-reducing archaeon from a deep-sea hydrothermal vent. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A hyperthermophilic, sulfur-reducing, organo-heterotrophic archaeon, strain OGL-20P(T), was isolated from 'black smoker' chimney material from the Rainbow hydrothermal vent site on the Mid-Atlantic Ridge (36.2 degrees N, 33.9 degrees W). The cells of strain OGL-20P(T) have an irregular coccoid shape and are motile with a single flagellum. Growth was observed within a pH range of 5.0-8.5 (optimum pH 7.0), an NaCl concentration range of 1-5 % (w/v) (optimum 3 %) and a temperature range of 55-94 degrees C (optimum 83-85 degrees C). The novel isolate is strictly anaerobic and obligately dependent upon elemental sulfur as an electron acceptor, but it does not reduce sulfate, sulfite, thiosulfate, Fe(III) or nitrate. Proteolysis products (peptone, bacto-tryptone, Casamino acids and yeast extract) are utilized as substrates during sulfur reduction. Strain OGL-20P(T) is resistant to ampicillin, chloramphenicol, kanamycin and gentamicin, but sensitive to tetracycline and rifampicin. The G+C content of the DNA is 52.9 mol%. The 16S rRNA gene sequence analysis revealed that strain OGL-20P(T) is closely related to Thermococcus coalescens and related species, but no significant homology by DNA-DNA hybridization was observed between those species and the new isolate. On the basis of physiological and molecular properties of the new isolate, we conclude that strain OGL-20P(T) represents a new separate species within the genus Thermococcus, for which we propose the name Thermococcus thioreducens sp. nov. The type strain is OGL-20P(T) (=JCM 12859(T)=DSM 14981(T)=ATCC BAA-394(T)). |
| 巻・号 | 57(Pt 7) |
| ページ | 1612-1618 |
| 公開日 | 2007-7-1 |
| DOI | 10.1099/ijs.0.65057-0 |
| PMID | 17625204 |
| MeSH | Anaerobiosis Anti-Bacterial Agents / pharmacology Atlantic Ocean Base Composition DNA, Archaeal / chemistry DNA, Archaeal / isolation & purification DNA, Ribosomal / chemistry DNA, Ribosomal / isolation & purification Ferric Compounds / metabolism Flagella Genes, rRNA Hot Springs / microbiology* Hydrogen-Ion Concentration Locomotion Molecular Sequence Data Nitrates / metabolism Nucleic Acid Hybridization Oxidation-Reduction Peptones / metabolism Phylogeny RNA, Archaeal / genetics RNA, Ribosomal, 16S / genetics Seawater / microbiology* Sequence Analysis, DNA Sequence Homology, Nucleic Acid Sodium Chloride / metabolism Sulfur / metabolism* Temperature Thermococcus / classification* Thermococcus / drug effects Thermococcus / isolation & purification* Thermococcus / physiology |
| IF | 2.415 |
| 引用数 | 33 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM8558 JCM12540 JCM11826 JCM12859 JCM |