RRC ID 53144
著者 Minami A, Tominaga Y, Furuto A, Kondo M, Kawamura Y, Uemura M.
タイトル Arabidopsis dynamin-related protein 1E in sphingolipid-enriched plasma membrane domains is associated with the development of freezing tolerance.
ジャーナル Plant J
Abstract The freezing tolerance of Arabidopsis thaliana is enhanced by cold acclimation, resulting in changes in the compositions and function of the plasma membrane. Here, we show that a dynamin-related protein 1E (DRP1E), which is thought to function in the vesicle trafficking pathway in cells, is related to an increase in freezing tolerance during cold acclimation. DRP1E accumulated in sphingolipid and sterol-enriched plasma membrane domains after cold acclimation. Analysis of drp1e mutants clearly showed that DRP1E is required for full development of freezing tolerance after cold acclimation. DRP1E fused with green fluorescent protein was visible as small foci that overlapped with fluorescent dye-labelled plasma membrane, providing evidence that DRP1E localizes non-uniformly in specific areas of the plasma membrane. These results suggest that DRP1E accumulates in sphingolipid and sterol-enriched plasma membrane domains and plays a role in freezing tolerance development during cold acclimation.
巻・号 83(3)
ページ 501-14
公開日 2015-8-1
DOI 10.1111/tpj.12907
PMID 26095877
MeSH Acclimatization / physiology* Arabidopsis / growth & development* Arabidopsis Proteins Cell Membrane / metabolism* Cold Shock Proteins and Peptides / physiology* GTP Phosphohydrolases Sphingolipids
IF 6.141
引用数 9
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda06484