論文 - 詳細
| RRC ID | 53162 |
|---|---|
| 著者 | Joh K, Matsuhisa F, Kitajima S, Nishioka K, Higashimoto K, Yatsuki H, Kono T, Koseki H, Soejima H. |
| タイトル | Growing oocyte-specific transcription-dependent de novo DNA methylation at the imprinted Zrsr1-DMR. |
| ジャーナル | Epigenetics Chromatin |
| Abstract |
BACKGROUND:Zrsr1 is a paternally expressed imprinted gene located in the first intron of Commd1, and the Zrsr1 promoter resides in a differentially methylated region (DMR) that is maternally methylated in the oocyte. However, a mechanism for the establishment of the methylation has remained obscure. Commd1 is transcribed in the opposite direction to Zrsr1 with predominant maternal expression, especially in the adult brain. RESULTS:We found Commed1 transcribed through the DMR in the growing oocyte. Zrsr1-DMR methylation was abolished by the prevention of Commd1 transcription. Furthermore, methylation did not occur at the artificially unmethylated maternal Zrsr1-DMR during embryonic development when transcription through the DMR was restored in the zygote. Loss of methylation at the maternal Zrsr1-DMR resulted in biallelic Zrsr1 expression and reduced the extent of the predominant maternal expression of Commd1. CONCLUSIONS:These results indicate that the establishment of methylation at Zrsr1-DMR occurs in a transcription-dependent and oocyte-specific manner and caused Zrsr1 imprinting by repressing maternal expression. The predominant maternal expression of Commd1 is likely caused by transcriptional interference by paternal Zrsr1 expression. |
| 巻・号 | 11(1) |
| ページ | 28 |
| 公開日 | 2018-6-6 |
| DOI | 10.1186/s13072-018-0200-6 |
| PII | 10.1186/s13072-018-0200-6 |
| PMID | 29875017 |
| PMC | PMC5989421 |
| MeSH | Adaptor Proteins, Signal Transducing / genetics* Animals DNA Methylation* Female Gene Expression Regulation, Developmental Genomic Imprinting Mice Nerve Tissue Proteins / genetics* Nuclear Proteins / genetics* Oocytes / chemistry Oocytes / growth & development* Organ Specificity Pregnancy Promoter Regions, Genetic Ribonucleoproteins / genetics* Splicing Factor U2AF Transcription, Genetic* |
| IF | 4.237 |
| 引用数 | 5 |
| リソース情報 | |
| 実験動物マウス | RBRC06155 |