| RRC ID |
5320
|
| 著者 |
Kibe R, Sakamoto M, Yokota H, Benno Y.
|
| タイトル |
Characterization of the inhabitancy of mouse intestinal bacteria (MIB) in rodents and humans by real-time PCR with group-specific primers.
|
| ジャーナル |
Microbiol Immunol
|
| Abstract |
Mouse intestinal bacteria (MIB) is a new operational taxonomic unit (OTU) belonging to the Bacteroides subgroup in the Cytophaga-Flavobacter-Bacteroides (CFB) phylum recently found in the intestine of mice, rats and humans. However, their characters are still unknown since they have not yet been isolated by culture. To understand their habitat characteristics in intestinal tracts, the quantification assays of MIB were established using MIB group-specific primers. The MIB population in the intestine was evaluated as a percentage of the number of 16S rRNA gene copy of MIB. A real-time PCR assay using group specific primers showed the fluctuation of MIB inhabitancy and revealed that the MIB population in the small intestine of mice was significantly lower than the large intestinal contents. Moreover, MIB was found in human feces though the number was lower than in murine. This assay using group-specific primers revealed new information about host-preference of MIB.
|
| 巻・号 |
51(4)
|
| ページ |
349-57
|
| 公開日 |
2007-1-1
|
| DOI |
10.1111/j.1348-0421.2007.tb03916.x
|
| PII |
JST.JSTAGE/mandi/51.349
|
| PMID |
17446674
|
| MeSH |
Animals
Bacteria / classification*
Bacteria / genetics
DNA Primers
Humans
Intestines / microbiology*
Mice
Mice, Inbred C57BL
Phylogeny
Polymerase Chain Reaction
RNA, Ribosomal, 16S / analysis
RNA, Ribosomal, 16S / genetics*
Rats
Rodentia
|
| IF |
1.566
|
| 引用数 |
11
|
|
WOS 分野
|
IMMUNOLOGY
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM11019
JCM5826
JCM10556
JCM5825
JCM6326
JCM2053 |
