RRC ID 53227
Author Yogo Y, Guo J, Iida T, Satoh K, Taguchi F, Takahashi H, Hall WW, Nagashima K.
Title Occurrence of multiple JC virus variants with distinctive regulatory sequences in the brain of a single patient with progressive multifocal leukoencephalopathy.
Journal Virus Genes
Abstract We established 99 JC virus (JCV) DNA clones directly from the brain of a single patient with progressive multifocal leukoencephalopathy (PML). Based upon restriction patterns, the cloned viral DNAs were classified into two major groups (NY-1A and -1B) containing 53 and 35 clones, respectively, and several minor groups containing one or a few clones. The regulatory sequences of representative clones were compared with the archetypal regulatory sequence, which has been detected in JCV DNAs cloned from the urine of healthy and nonimmunosuppressed individuals. The regulatory sequence of NY-1B had the two structural features common to most PML-type regulatory regions, duplication and deletion of specific segments in the archetypal sequence, while that of NY-1A contained a small deletion and an insertion of a 29-bp sequence originating from the early region of the JCV genome. A regulatory region similar to that of NY-1A has never been detected in JCV isolates obtained thus far.
Volume 8(2)
Pages 99-105
Published 1994-3-1
DOI 10.1007/BF01703608
PMID 7915447
MeSH AIDS-Related Opportunistic Infections / complications Adult Base Sequence Brain / microbiology* Capsid / genetics Capsid Proteins* Cloning, Molecular Genes, Viral / genetics Genetic Variation / genetics* Humans JC Virus / genetics* Leukoencephalopathy, Progressive Multifocal / complications Leukoencephalopathy, Progressive Multifocal / microbiology* Male Molecular Sequence Data Polymorphism, Restriction Fragment Length Regulatory Sequences, Nucleic Acid / genetics* Restriction Mapping
IF 1.616
Times Cited 30
DNA material pNY-1A (RDB07250) pNY-1B (RDB07251).