RRC ID 53228
Author Takahashi H, Yogo Y, Furuta Y, Takada A, Irie T, Kasai M, Sano K, Fujioka Y, Nagashima K.
Title Molecular characterization of a JC virus (Sap-1) clone derived from a cerebellar form of progressive multifocal leukoencephalopathy.
Journal Acta Neuropathol
Abstract Progressive multifocal leukoencephalopathy (PML) is a demyelinating disease caused by polyomavirus JC (JCV). In the majority of cases of PML the cerebrum is mainly affected (cerebral PML) but on rare occasions lesions are restricted to the cerebellum and brain stem (cerebellar PML). We report a rare cerebellar PML case which occurred in a Japanese patient undergoing prolonged hemodialysis treatment. To understand the molecular basis of the viral tissue tropism, we molecularly cloned JCV DNA and compared it with those of cerebral PML. Of ten clones analyzed nine showed identical fragment patterns after digestion with various restriction endonucleases, and we designated these clones Sap-1. It could be shown that the basic structures of the regulatory regions are similar between Sap-1 and isolates from cerebral PML. Restriction endonuclease mapping analysis was used to examine the genetic relationship between Sap-1 and urine-derived isolates containing the archetypal regulatory sequence. We found that Sap-1 was genetically related to an archetypal JCV isolate in Japan.
Volume 83(2)
Pages 105-12
Published 1992-1-1
DOI 10.1007/BF00308469
PMID 1313631
MeSH Base Sequence Cerebellar Diseases / microbiology* Cerebellar Diseases / pathology Cerebellum / microbiology Cerebellum / pathology Cloning, Molecular DNA Restriction Enzymes DNA, Viral / chemistry Female Humans Immunohistochemistry JC Virus / chemistry* JC Virus / genetics Kidney Failure, Chronic / complications Kidney Failure, Chronic / pathology Kidney Failure, Chronic / therapy Leukoencephalopathy, Progressive Multifocal / microbiology* Leukoencephalopathy, Progressive Multifocal / pathology Microscopy, Electron Middle Aged Molecular Sequence Data Renal Dialysis Tumor Virus Infections / microbiology* Tumor Virus Infections / pathology
IF 14.256
Times Cited 23
DNA material pSap-1 clone1 (RDB07249)