論文 - 詳細
| RRC ID | 53235 |
|---|---|
| 著者 | Zhang Y, Wang H, Zhang J, Hu Y, Zhang L, Wu X, Su X, Li T, Zou X, Liang B. |
| タイトル | The cytochrome b5 reductase HPO-19 is required for biosynthesis of polyunsaturated fatty acids in Caenorhabditis elegans. |
| ジャーナル | Biochim Biophys Acta |
| Abstract |
Polyunsaturated fatty acids (PUFAs) are fatty acids with backbones containing more than one double bond, which are introduced by a series of desaturases that insert double bonds at specific carbon atoms in the fatty acid chain. It has been established that desaturases need flavoprotein-NADH-dependent cytochrome b5 reductase (simplified as cytochrome b5 reductase) and cytochrome b5 to pass through electrons for activation. However, it has remained unclear how this multi-enzyme system works for distinct desaturases. The model organism Caenorhabditis elegans contains seven desaturases (FAT-1, -2, -3, -4, -5, -6, -7) for the biosynthesis of PUFAS, providing an excellent model in which to characterize different desaturation reactions. Here, we show that RNAi inactivation of predicted cytochrome b5 reductases hpo-19 and T05H4.4 led to increased levels of C18:1n-9 but decreased levels of PUFAs, small lipid droplets, decreased fat accumulation, reduced brood size and impaired development. Dietary supplementation with different fatty acids showed that HPO-19 and T05H4.4 likely affect the activity of FAT-1, FAT-2, FAT-3, and FAT-4 desaturases, suggesting that these four desaturases use the same cytochrome b5 reductase to function. Collectively, these findings indicate that cytochrome b5 reductase HPO-19/T05H4.4 is required for desaturation to biosynthesize PUFAs in C. elegans. |
| 巻・号 | 1861(4) |
| ページ | 310-9 |
| 公開日 | 2016-4-1 |
| DOI | 10.1016/j.bbalip.2016.01.009 |
| PII | S1388-1981(16)00010-X |
| PMID | 26806391 |
| MeSH | Animals Animals, Genetically Modified Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cytochrome-B(5) Reductase / genetics Cytochrome-B(5) Reductase / metabolism* Fatty Acid Desaturases / genetics Fatty Acid Desaturases / metabolism* Fatty Acids, Unsaturated / biosynthesis* Lipid Droplets / metabolism Reproduction Time Factors |
| IF | 3.411 |
| 引用数 | 7 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | |