| RRC ID |
5325
|
| 著者 |
Burns DG, Janssen PH, Itoh T, Kamekura M, Li Z, Jensen G, Rodríguez-Valera F, Bolhuis H, Dyall-Smith ML.
|
| タイトル |
Haloquadratum walsbyi gen. nov., sp. nov., the square haloarchaeon of Walsby, isolated from saltern crystallizers in Australia and Spain.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
Strains C23T and HBSQ001 were isolated from solar salterns and are novel square-shaped, aerobic, extremely halophilic members of the domain Archaea and family Halobacteriaceae. Cells stained Gram-negative and grew optimally in media containing 18 % salts at around neutral pH. Mg2+ is not required. The DNA G+C content of both isolates was 46.9 mol% and DNA-DNA cross-hybridization showed a relatedness of 80 %. Their 16S rRNA gene sequences showed only 2 nucleotide differences (99.9 % identity) and phylogenetic tree reconstructions with other recognized members of the Halobacteriaceae indicated that they formed a distinct clade, with the closest relative being Halogeometricum borinquense PR 3T (91.2 % sequence identity). The major polar glycolipid of both isolates was the sulfated diglycosyl diether lipid S-DGD-1. Electron cryomicrosopy of whole cells revealed similar internal structures, such as gas vesicles and polyhydroxyalkanoate granules, but the cell wall of isolate HBSQ001 displayed a more complex S-layer compared with that of isolate C23T. The phenotypic characterization and phylogenetic data support the placement of isolates C23T and HBSQ001 in a novel species in a new genus within the Halobacteriaceae, for which we propose the name Haloquadratum walsbyi gen. nov., sp. nov. The type strain of Haloquadratum walsbyi is C23T (=JCM 12705T=DSM 16854T).
|
| 巻・号 |
57(Pt 2)
|
| ページ |
387-392
|
| 公開日 |
2007-2-1
|
| DOI |
10.1099/ijs.0.64690-0
|
| PMID |
17267984
|
| MeSH |
Aerobiosis
Australia
Base Composition
Cell Wall / ultrastructure
Cytoplasm / ultrastructure
DNA, Archaeal / chemistry
DNA, Archaeal / isolation & purification
Genes, rRNA
Halobacteriaceae / classification*
Halobacteriaceae / cytology
Halobacteriaceae / isolation & purification*
Halobacteriaceae / physiology
Hydrogen-Ion Concentration
Magnesium / metabolism
Molecular Sequence Data
Nucleic Acid Hybridization
Organelles / ultrastructure
Phospholipids / analysis
Phospholipids / chemistry
Phylogeny
RNA, Archaeal / genetics
RNA, Ribosomal, 16S / genetics
Salts
Seawater / microbiology*
Sequence Analysis, DNA
Spain
Water Microbiology
|
| IF |
2.415
|
| 引用数 |
101
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM10706
JCM11081
JCM9101
JCM12705
JCM12895 |
