RRC ID 5327
Author Odamaki T, Xiao JZ, Iwabuchi N, Sakamoto M, Takahashi N, Kondo S, Iwatsuki K, Kokubo S, Togashi H, Enomoto T, Benno Y.
Title Fluctuation of fecal microbiota in individuals with Japanese cedar pollinosis during the pollen season and influence of probiotic intake.
Journal J Investig Allergol Clin Immunol
Abstract BACKGROUND:We have previously reported the results of a randomized, double-blind, placebo-controlled trial that found the intake of yogurt supplemented with a probiotic strain, Bifidobacterium longum BB536, alleviates symptoms and affects blood parameters in individuals with Japanese cedar pollinosis (JCPsis) during the pollen season.
OBJECTIVE:In the present study, fecal microbiota were investigated to examine whether any changes occur during the pollen season and whether any influence is exerted by probiotic intake.
METHODS:Yogurt either with BB536 (BB536 yogurt) or without BB536 (placebo yogurt) was administered for 14 weeks at 2 x 100 g per day to 40 subjects (17 men, 23 women) with a clinical history of JCPsis. Fecal samples were obtained from 23 subjects (placebo group, n=13; BB536 group, n=10) before and during the intervention (weeks 4, 9 and 13) and fecal microbiota were analyzed using terminal-restriction fragment length polymorphism and real-time polymerase chain reaction (PCR) methods.
RESULTS:From the fluctuation patterns of terminal-restriction fragments, the Bacteroides fragilis group and bifidobacteria were among the species that changed most with pollen dispersion. Real-time PCR analyses indicated that the cell numbers of the B fragilis group increased significantly along with pollen dispersion in both BB536 and placebo groups. Cell numbers of bifidobacteria were significantly higher in the BB536 group compared with the placebo group (P < .05 at weeks 4 and 9). The ratio of cell numbers of the B fragilis group to bifidobacteria increased significantly during the pollen season in the placebo group (P < .01 at weeks 9 and 14), but not in the BB536 group. An in vitro study using peripheral blood mononuclear cells from JCPsis subjects indicated that strains of the B fragilis group induced significantly more helper T cell (T(H)) type2 cytokines (interleukin [IL]-6) but fewer T(H)1 cytokines (IL-12 and interferon) compared with those of bifidobacteria.
CONCLUSIONS:These results suggest a relationship between fluctuation in intestinal microbiota and pollinosis allergy. Furthermore, intake of BB536 yogurt appears to exert positive ihfluences on the formation of anti-allergic microbiota.
Volume 17(2)
Pages 92-100
Published 2007
PMID 17460947
MeSH Adolescent Adult Bifidobacterium / immunology* Bifidobacterium / isolation & purification Bifidobacterium / metabolism Colony Count, Microbial Cryptomeria / immunology* Eosinophilia / blood Eosinophilia / classification Feces / microbiology* Female Humans Interferon-gamma / blood Leukocyte Count Male Middle Aged Probiotics / administration & dosage* Probiotics / metabolism Rhinitis, Allergic, Seasonal / immunology* Rhinitis, Allergic, Seasonal / microbiology Rhinitis, Allergic, Seasonal / therapy Yogurt / microbiology*
IF 3.457
Times Cited 25
WOS Category ALLERGY IMMUNOLOGY
Resource
General Microbes JCM1217 JCM5826 JCM1275 JCM1194 JCM1200 JCM7041 JCM7135 JCM11019 JCM5824 JCM5825 JCM5827 JCM5828 JCM9496 JCM9497 JCM9498