RRC ID 53286
著者 Imae R, Dejima K, Kage-Nakadai E, Arai H, Mitani S.
タイトル Endomembrane-associated RSD-3 is important for RNAi induced by extracellular silencing RNA in both somatic and germ cells of Caenorhabditis elegans.
ジャーナル Sci Rep
Abstract RNA silencing signals in C. elegans spread among cells, leading to RNAi throughout the body. During systemic spread of RNAi, membrane trafficking is thought to play important roles. Here, we show that RNAi Spreading Defective-3 (rsd-3), which encodes a homolog of epsinR, a conserved ENTH (epsin N-terminal homology) domain protein, generally participates in cellular uptake of silencing RNA. RSD-3 is previously thought to be involved in systemic RNAi only in germ cells, but we isolated several deletion alleles of rsd-3, and found that these mutants are defective in the spread of silencing RNA not only into germ cells but also into somatic cells. RSD-3 is ubiquitously expressed, and intracellularly localized to the trans-Golgi network (TGN) and endosomes. Tissue-specific rescue experiments indicate that RSD-3 is required for importing silencing RNA into cells rather than exporting from cells. Structure/function analysis showed that the ENTH domain alone is sufficient, and membrane association of the ENTH domain is required, for RSD-3 function in systemic RNAi. Our results suggest that endomembrane trafficking through the TGN and endosomes generally plays an important role in cellular uptake of silencing RNA.
巻・号 6
ページ 28198
公開日 2016-6-16
DOI 10.1038/srep28198
PII srep28198
PMID 27306325
PMC PMC4910058
MeSH Adaptor Proteins, Vesicular Transport / genetics* Animals Biological Transport / genetics Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics* Cell Communication / genetics Germ Cells / metabolism Protein Domains / genetics RNA Interference / physiology* RNA, Small Interfering / genetics* Repressor Proteins / genetics* Transgenes / genetics
IF 3.998
引用数 2
リソース情報
線虫 tm9006 tm5404 tm5659 tm1447 tm1703 tm2622 tm6130 tm6188 tm5041 tm2124