Reference - Detail
| RRC ID | 53300 |
|---|---|
| Author | Todd PA, McCue HV, Haynes LP, Barclay JW, Burgoyne RD. |
| Title | Interaction of ARF-1.1 and neuronal calcium sensor-1 in the control of the temperature-dependency of locomotion in Caenorhabditis elegans. |
| Journal | Sci Rep |
| Abstract |
Neuronal calcium sensor-1 (NCS-1) mediates changes in cellular function by regulating various target proteins. Many potential targets have been identified but the physiological significance of only a few has been established. Upon temperature elevation, Caenorhabditis elegans exhibits reversible paralysis. In the absence of NCS-1, worms show delayed onset and a shorter duration of paralysis. This phenotype can be rescued by re-expression of ncs-1 in AIY neurons. Mutants with defects in four potential NCS-1 targets (arf-1.1, pifk-1, trp-1 and trp-2) showed qualitatively similar phenotypes to ncs-1 null worms, although the effect of pifk-1 mutation on time to paralysis was considerably delayed. Inhibition of pifk-1 also resulted in a locomotion phenotype. Analysis of double mutants showed no additive effects between mutations in ncs-1 and trp-1 or trp-2. In contrast, double mutants of arf-1.1 and ncs-1 had an intermediate phenotype, consistent with NCS-1 and ARF-1.1 acting in the same pathway. Over-expression of arf-1.1 in the AIY neurons was sufficient to rescue partially the phenotype of both the arf-1.1 and the ncs-1 null worms. These findings suggest that ARF-1.1 interacts with NCS-1 in AIY neurons and potentially pifk-1 in the Ca(2+) signaling pathway that leads to inhibited locomotion at an elevated temperature. |
| Volume | 6 |
| Pages | 30023 |
| Published | 2016-7-20 |
| DOI | 10.1038/srep30023 |
| PII | srep30023 |
| PMID | 27435667 |
| PMC | PMC4951722 |
| MeSH | ADP-Ribosylation Factor 1 / metabolism* Animals Caenorhabditis elegans / physiology* Caenorhabditis elegans / radiation effects* Caenorhabditis elegans Proteins / metabolism* Locomotion / radiation effects Neuronal Calcium-Sensor Proteins / metabolism* Neuropeptides / metabolism* Temperature |
| IF | 3.998 |
| Times Cited | 3 |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm2348 |