論文 - 詳細
| RRC ID | 53352 |
|---|---|
| 著者 | Takayanagi-Kiya S, Zhou K, Jin Y. |
| タイトル | Release-dependent feedback inhibition by a presynaptically localized ligand-gated anion channel. |
| ジャーナル | Elife |
| Abstract |
Presynaptic ligand-gated ion channels (LGICs) have long been proposed to affect neurotransmitter release and to tune the neural circuit activity. However, the understanding of their in vivo physiological action remains limited, partly due to the complexity in channel types and scarcity of genetic models. Here we report that C. elegans LGC-46, a member of the Cys-loop acetylcholine (ACh)-gated chloride (ACC) channel family, localizes to presynaptic terminals of cholinergic motor neurons and regulates synaptic vesicle (SV) release kinetics upon evoked release of acetylcholine. Loss of lgc-46 prolongs evoked release, without altering spontaneous activity. Conversely, a gain-of-function mutation of lgc-46 shortens evoked release to reduce synaptic transmission. This inhibition of presynaptic release requires the anion selectivity of LGC-46, and can ameliorate cholinergic over-excitation in a C. elegans model of excitation-inhibition imbalance. These data demonstrate a novel mechanism of presynaptic negative feedback in which an anion-selective LGIC acts as an auto-receptor to inhibit SV release. |
| 巻・号 | 5 |
| 公開日 | 2016-10-26 |
| DOI | 10.7554/eLife.21734 |
| PII | e21734 |
| PMID | 27782882 |
| PMC | PMC5102579 |
| MeSH | Animals Caenorhabditis elegans / enzymology* Caenorhabditis elegans Proteins / metabolism* Chloride Channels / metabolism* Cholinergic Neurons / enzymology* Evoked Potentials Feedback, Physiological* Motor Neurons / enzymology* Neurotransmitter Agents / metabolism Presynaptic Terminals / enzymology* |
| IF | 7.08 |
| 引用数 | 8 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3268 tm3174 tm6556 |