論文 - 詳細
| RRC ID | 53394 |
|---|---|
| 著者 | Sugioka K, Hamill DR, Lowry JB, McNeely ME, Enrick M, Richter AC, Kiebler LE, Priess JR, Bowerman B. |
| タイトル | Centriolar SAS-7 acts upstream of SPD-2 to regulate centriole assembly and pericentriolar material formation. |
| ジャーナル | Elife |
| Abstract |
The centriole/basal body is a eukaryotic organelle that plays essential roles in cell division and signaling. Among five known core centriole proteins, SPD-2/Cep192 is the first recruited to the site of daughter centriole formation and regulates the centriolar localization of the other components in C. elegans and in humans. However, the molecular basis for SPD-2 centriolar localization remains unknown. Here, we describe a new centriole component, the coiled-coil protein SAS-7, as a regulator of centriole duplication, assembly and elongation. Intriguingly, our genetic data suggest that SAS-7 is required for daughter centrioles to become competent for duplication, and for mother centrioles to maintain this competence. We also show that SAS-7 binds SPD-2 and regulates SPD-2 centriolar recruitment, while SAS-7 centriolar localization is SPD-2-independent. Furthermore, pericentriolar material (PCM) formation is abnormal in sas-7 mutants, and the PCM-dependent induction of cell polarity that defines the anterior-posterior body axis frequently fails. We conclude that SAS-7 functions at the earliest step in centriole duplication yet identified and plays important roles in the orchestration of centriole and PCM assembly. |
| 巻・号 | 6 |
| 公開日 | 2017-1-16 |
| DOI | 10.7554/eLife.20353 |
| PII | e20353 |
| PMID | 28092264 |
| PMC | PMC5342823 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / metabolism* Cell Cycle Proteins / metabolism* Centrioles / metabolism* Humans Organelle Biogenesis* |
| IF | 7.08 |
| 引用数 | 20 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 28 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1105 |