論文 - 詳細
| RRC ID | 53420 |
|---|---|
| 著者 | Janssen IJ, Krücken J, Demeler J, von Samson-Himmelstjerna G. |
| タイトル | Transgenically expressed Parascaris P-glycoprotein-11 can modulate ivermectin susceptibility in Caenorhabditis elegans. |
| ジャーナル | Int J Parasitol Drugs Drug Resist |
| Abstract |
P-glycoproteins (Pgps) are suspected to mediate drug extrusion in nematodes contributing to macrocyclic lactone resistance. This association was recently shown for Parascaris Pgp-11. Ivermectin resistance was correlated with the presence of three pgp-11 single nucleotide polymorphisms and/or increased pgp-11 mRNA levels. In the present study, the ability of Pgp-11 to modulate ivermectin susceptibility was investigated by its expression in a pgp-11-deficient Caenorhabditis elegans strain. Expression of Parascaris pgp-11 in two transgenic lines significantly decreased ivermectin susceptibility in a motility (thrashing) assay conducted in liquid medium. The EC50 values increased by 3.2- and 4.6-fold in the two lines relative to a transgenic control strain. This is the first report on the successful functional analysis of a parasitic nematode Pgp in the model organism C. elegans. |
| 巻・号 | 5(2) |
| ページ | 44-7 |
| 公開日 | 2015-8-1 |
| DOI | 10.1016/j.ijpddr.2015.03.003 |
| PII | S2211-3207(15)00006-8 |
| PMID | 25905032 |
| PMC | PMC4401813 |
| MeSH | ATP Binding Cassette Transporter, Subfamily B / genetics ATP Binding Cassette Transporter, Subfamily B / metabolism* Animals Animals, Genetically Modified Antiparasitic Agents / pharmacology Ascaridoidea / genetics Ascaridoidea / metabolism* Caenorhabditis elegans / drug effects* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Drug Resistance* Gene Expression Regulation Ivermectin / pharmacology* |
| IF | 3.03 |
| 引用数 | 19 |
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