RRC ID 53431
Author Nelson MD, Janssen T, York N, Lee KH, Schoofs L, Raizen DM.
Title FRPR-4 Is a G-Protein Coupled Neuropeptide Receptor That Regulates Behavioral Quiescence and Posture in Caenorhabditis elegans.
Journal PLoS One
Abstract Neuropeptides signal through G-protein coupled receptors (GPCRs) to regulate a broad array of animal behaviors and physiological processes. The Caenorhabditis elegans genome encodes approximately 100 predicted neuropeptide receptor GPCRs, but in vivo roles for only a few have been identified. We describe here a role for the GPCR FRPR-4 in the regulation of behavioral quiescence and locomotive posture. FRPR-4 is activated in cell culture by several neuropeptides with an amidated isoleucine-arginine-phenylalanine (IRF) motif or an amidated valine-arginine-phenylalanine (VRF) motif at their carboxy termini, including those encoded by the gene flp-13. Loss of frpr-4 function results in a minor feeding quiescence defect after heat-induced cellular stress. Overexpression of frpr-4 induces quiescence of locomotion and feeding as well as an exaggerated body bend posture. The exaggerated body bend posture requires the gene flp-13. While frpr-4 is expressed broadly, selective overexpression of frpr-4 in the proprioceptive DVA neurons results in exaggerated body bends that require flp-13 in the ALA neuron. Our results suggest that FLP-13 and other neuropeptides signal through FRPR-4 and other receptors to regulate locomotion posture and behavioral quiescence.
Volume 10(11)
Pages e0142938
Published 2015-1-1
DOI 10.1371/journal.pone.0142938
PII PONE-D-15-36196
PMID 26571132
PMC PMC4646455
MeSH Amino Acid Sequence Animals Behavior, Animal* Caenorhabditis elegans / drug effects Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism* Drosophila melanogaster / metabolism Heat-Shock Response / drug effects Locomotion / drug effects Molecular Sequence Data Neurons / drug effects Neurons / metabolism Peptides / chemistry Peptides / pharmacology Posture Receptors, G-Protein-Coupled / metabolism* Receptors, Neuropeptide / metabolism*
IF 2.776
Times Cited 7