論文 - 詳細
| RRC ID | 53438 |
|---|---|
| 著者 | Nishimura H, Tajima T, Comstra HS, Gleason EJ, L'Hernault SW. |
| タイトル | The Immunoglobulin-like Gene spe-45 Acts during Fertilization in Caenorhabditis elegans like the Mouse Izumo1 Gene. |
| ジャーナル | Curr Biol |
| Abstract |
The Caenorhabditis elegans spe-9 class genes, which show specific or predominant expression in the male germline, are indispensable for fertilization [1, 2]. However, due to the rapid evolution of genes involved in reproduction, we do not currently know if there are spe-9 class genes in mammals that play similar roles during fertilization to those found in C. elegans. In mice, the Izumo1 gene encodes a sperm-specific transmembrane (TM) protein with a single immunoglobulin (Ig)-like domain that is absolutely required for gamete fusion [3, 4]. In this study, we hypothesized that C. elegans has a new member of the spe-9 class genes coding for an IZUMO1-like protein. We screened C. elegans microarray data [5, 6] to identify male germline-enriched genes that encode membrane proteins with Ig-like domains. A deletion (tm3715) in one such gene (F28D1.8) caused hermaphrodites to show a male germline-dependent self-sterility, so we have named it spe-45. Mutant spe-45 worms seemed to normally undergo spermatogenesis (spermatid production by meiosis) and spermiogenesis (spermatid activation into actively motile spermatozoa). spe-45 mutant spermatozoa, however, could not complete gamete fusion, which is a characteristic of all spe-9 class mutants [1, 2]. Moreover, spe-45 self-sterile worms were rescued by a transgene expressing chimeric SPE-45 protein in which its Ig-like domain was replaced by the Ig-like domain from mouse IZUMO1. Hence, C. elegans SPE-45 and mouse IZUMO1 appear to have retained a common function(s) that is required during fertilization. |
| 巻・号 | 25(24) |
| ページ | 3225-31 |
| 公開日 | 2015-12-21 |
| DOI | 10.1016/j.cub.2015.10.056 |
| PII | S0960-9822(15)01350-0 |
| PMID | 26671669 |
| PMC | PMC4691390 |
| MeSH | Amino Acid Sequence Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics* Female Fertilization* Immunoglobulins / genetics* Male Membrane Proteins / genetics* Mice Molecular Sequence Data Spermatogenesis |
| IF | 9.601 |
| 引用数 | 6 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2421 tm3715 |