RRC ID 53469
著者 Essig YJ, Webb SM, Stürzenbaum SR.
タイトル Deletion of Phytochelatin Synthase Modulates the Metal Accumulation Pattern of Cadmium Exposed C. elegans.
ジャーナル Int J Mol Sci
Abstract Environmental metal pollution is a growing health risk to flora and fauna. It is therefore important to fully elucidate metal detoxification pathways. Phytochelatin synthase (PCS), an enzyme involved in the biosynthesis of phytochelatins (PCs), plays an important role in cadmium detoxification. The PCS and PCs are however not restricted to plants, but are also present in some lower metazoans. The model nematode Caenorhabditis elegans, for example, contains a fully functional phytochelatin synthase and phytochelatin pathway. By means of a transgenic nematode strain expressing a pcs-1 promoter-tagged GFP (pcs-1::GFP) and a pcs-1 specific qPCR assay, further evidence is presented that the expression of the C. elegans phytochelatin synthase gene (pcs-1) is transcriptionally non-responsive to a chronic (48 h) insult of high levels of zinc (500 μM) or acute (3 h) exposures to high levels of cadmium (300 μM). However, the accumulation of cadmium, but not zinc, is dependent on the pcs-1 status of the nematode. Synchrotron based X-ray fluorescence imaging uncovered that the cadmium body burden increased significantly in the pcs-1(tm1748) knockout allele. Taken together, this suggests that whilst the transcription of pcs-1 may not be mediated by an exposure zinc or cadmium, it is nevertheless an integral part of the cadmium detoxification pathway in C. elegans.
巻・号 17(2)
ページ 257
公開日 2016-2-19
DOI 10.3390/ijms17020257
PII ijms17020257
PMID 26907254
PMC PMC4783986
MeSH Aminoacyltransferases / genetics* Animals Animals, Genetically Modified Cadmium / analysis* Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics* Environmental Pollutants / analysis* Gene Deletion* Phytochelatins / biosynthesis Spectrometry, X-Ray Emission / instrumentation Synchrotrons Zinc / analysis
IF 4.556
引用数 8
リソース情報
線虫 tm1748