論文 - 詳細
| RRC ID | 53499 |
|---|---|
| 著者 | Müller A, Winkler J, Fiedler F, Sastradihardja T, Binder C, Schnabel R, Kungel J, Rothemund S, Hennig C, Schöneberg T, Prömel S. |
| タイトル | Oriented Cell Division in the C. elegans Embryo Is Coordinated by G-Protein Signaling Dependent on the Adhesion GPCR LAT-1. |
| ジャーナル | PLoS Genet |
| Abstract |
Orientation of spindles and cell division planes during development of many species ensures that correct cell-cell contacts are established, which is vital for proper tissue formation. This is a tightly regulated process involving a complex interplay of various signals. The molecular mechanisms underlying several of these pathways are still incompletely understood. Here, we identify the signaling cascade of the C. elegans latrophilin homolog LAT-1, an essential player in the coordination of anterior-posterior spindle orientation during the fourth round of embryonic cell division. We show that the receptor mediates a G protein-signaling pathway revealing that G-protein signaling in oriented cell division is not solely GPCR-independent. Genetic analyses showed that through the interaction with a Gs protein LAT-1 elevates intracellular cyclic AMP (cAMP) levels in the C. elegans embryo. Stimulation of this G-protein cascade in lat-1 null mutant nematodes is sufficient to orient spindles and cell division planes in the embryo in the correct direction. Finally, we demonstrate that LAT-1 is activated by an intramolecular agonist to trigger this cascade. Our data support a model in which a novel, GPCR-dependent G protein-signaling cascade mediated by LAT-1 controls alignment of cell division planes in an anterior-posterior direction via a metabotropic Gs-protein/adenylyl cyclase pathway by regulating intracellular cAMP levels. |
| 巻・号 | 11(10) |
| ページ | e1005624 |
| 公開日 | 2015-10-1 |
| DOI | 10.1371/journal.pgen.1005624 |
| PII | PGENETICS-D-15-01575 |
| PMID | 26505631 |
| PMC | PMC4624771 |
| MeSH | Adenylyl Cyclases / genetics Adenylyl Cyclases / metabolism Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development Cell Adhesion / genetics Cell Division / genetics* Cyclic AMP / genetics Embryo, Nonmammalian GTP-Binding Proteins / genetics GTP-Binding Proteins / metabolism* Large Neutral Amino Acid-Transporter 1 / genetics* Large Neutral Amino Acid-Transporter 1 / metabolism Signal Transduction |
| IF | 5.224 |
| 引用数 | 36 |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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