論文 - 詳細
| RRC ID | 53500 |
|---|---|
| 著者 | Palmisano NJ, Meléndez A. |
| タイトル | Detection of Autophagy in Caenorhabditis elegans. |
| ジャーナル | Cold Spring Harb Protoc |
| Abstract |
Autophagy is a dynamic and catabolic process that results in the breakdown and recycling of cellular components through the autophagosomal-lysosomal pathway. Many autophagy genes identified in yeasts and mammals have orthologs in the nematode Caenorhabditis elegans. In recent years, gene inactivation by RNA interference (RNAi) and chromosomal mutations has been useful to probe the functions of autophagy in C. elegans, and a role for autophagy has been shown to contribute to multiple processes, such as the adaptation to stress, longevity, cell death, cell growth control, clearance of aggregation-prone proteins, degradation of P granules during embryogenesis, and apoptotic cell clearance. Here, we discuss some of these roles and describe methods that can be used to study autophagy in C. elegans. Specifically, we summarize how to visualize autophagy in embryos, larva, or adults, how to detect the lipidation of the ubiquitin-like modifier LGG-1 by western blot, and how to inactivate autophagy genes by RNAi. |
| 巻・号 | 2016(2) |
| ページ | pdb.top070466 |
| 公開日 | 2016-2-1 |
| DOI | 10.1101/pdb.top070466 |
| PII | 2016/2/pdb.top070466 |
| PMID | 26832690 |
| MeSH | Animals Autophagy* Blotting, Western / methods Caenorhabditis elegans / physiology* Gene Knockdown Techniques Molecular Biology / methods* Optical Imaging / methods* Parasitology / methods* RNA Interference |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3949 tm3948 tm831 tm3489 |