論文 - 詳細
| RRC ID | 53509 |
|---|---|
| 著者 | Zhang F, Peng D, Cheng C, Zhou W, Ju S, Wan D, Yu Z, Shi J, Deng Y, Wang F, Ye X, Hu Z, Lin J, Ruan L, Sun M. |
| タイトル | Bacillus thuringiensis Crystal Protein Cry6Aa Triggers Caenorhabditis elegans Necrosis Pathway Mediated by Aspartic Protease (ASP-1). |
| ジャーナル | PLoS Pathog |
| Abstract |
Cell death plays an important role in host-pathogen interactions. Crystal proteins (toxins) are essential components of Bacillus thuringiensis (Bt) biological pesticides because of their specific toxicity against insects and nematodes. However, the mode of action by which crystal toxins to induce cell death is not completely understood. Here we show that crystal toxin triggers cell death by necrosis signaling pathway using crystal toxin Cry6Aa-Caenorhabditis elegans toxin-host interaction system, which involves an increase in concentrations of cytoplasmic calcium, lysosomal lyses, uptake of propidium iodide, and burst of death fluorescence. We find that a deficiency in the necrosis pathway confers tolerance to Cry6Aa toxin. Intriguingly, the necrosis pathway is specifically triggered by Cry6Aa, not by Cry5Ba, whose amino acid sequence is different from that of Cry6Aa. Furthermore, Cry6Aa-induced necrosis pathway requires aspartic protease (ASP-1). In addition, ASP-1 protects Cry6Aa from over-degradation in C. elegans. This is the first demonstration that deficiency in necrosis pathway confers tolerance to Bt crystal protein, and that Cry6A triggers necrosis represents a newly added necrosis paradigm in the C. elegans. Understanding this model could lead to new strategies for nematode control. |
| 巻・号 | 12(1) |
| ページ | e1005389 |
| 公開日 | 2016-1-1 |
| DOI | 10.1371/journal.ppat.1005389 |
| PII | PPATHOGENS-D-15-01711 |
| PMID | 26795495 |
| PMC | PMC4721865 |
| MeSH | Amino Acid Sequence Animals Aspartic Acid Proteases / chemistry Aspartic Acid Proteases / metabolism* Bacillus thuringiensis Toxins Bacterial Proteins / metabolism* Caenorhabditis elegans / enzymology* Calorimetry Electrophoresis, Gel, Two-Dimensional Endotoxins / metabolism* Enzyme-Linked Immunosorbent Assay Hemolysin Proteins / metabolism* Host-Pathogen Interactions Immunoblotting Mass Spectrometry Molecular Sequence Data Necrosis Pest Control, Biological / methods |
| IF | 6.218 |
| 引用数 | 22 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 13 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm4559 tm666 |