論文 - 詳細
| RRC ID | 53513 |
|---|---|
| 著者 | Chuang M, Hsiao TI, Tong A, Xu S, Chisholm AD. |
| タイトル | DAPK interacts with Patronin and the microtubule cytoskeleton in epidermal development and wound repair. |
| ジャーナル | Elife |
| Abstract |
Epidermal barrier epithelia form a first line of defense against the environment, protecting animals against infection and repairing physical damage. In C. elegans, death-associated protein kinase (DAPK-1) regulates epidermal morphogenesis, innate immunity and wound repair. Combining genetic suppressor screens and pharmacological tests, we find that DAPK-1 maintains epidermal tissue integrity through regulation of the microtubule (MT) cytoskeleton. dapk-1 epidermal phenotypes are suppressed by treatment with microtubule-destabilizing drugs and mimicked or enhanced by microtubule-stabilizing drugs. Loss of function in ptrn-1, the C. elegans member of the Patronin/Nezha/CAMSAP family of MT minus-end binding proteins, suppresses dapk-1 epidermal and innate immunity phenotypes. Over-expression of the MT-binding CKK domain of PTRN-1 triggers epidermal and immunity defects resembling those of dapk-1 mutants, and PTRN-1 localization is regulated by DAPK-1. DAPK-1 and PTRN-1 physically interact in co-immunoprecipitation experiments, and DAPK-1 itself undergoes MT-dependent transport. Our results uncover an unexpected interdependence of DAPK-1 and the microtubule cytoskeleton in maintenance of epidermal integrity. |
| 巻・号 | 5 |
| 公開日 | 2016-9-23 |
| DOI | 10.7554/eLife.15833 |
| PII | e15833 |
| PMID | 27661253 |
| PMC | PMC5053806 |
| MeSH | Animals Caenorhabditis elegans / physiology Caenorhabditis elegans Proteins / metabolism* Death-Associated Protein Kinases / metabolism* Epidermis / physiology* Microtubule-Associated Proteins / metabolism* Microtubules / metabolism* Protein Binding Protein Transport Wound Healing* |
| IF | 7.08 |
| 引用数 | 8 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm5597 tm2143 tm683 tm773 tm1357 tm3861 tm3124 tm3360 tm4059 tm4957 tm2235 |