論文 - 詳細
| RRC ID | 53521 |
|---|---|
| 著者 | Izumikawa T, Dejima K, Watamoto Y, Nomura KH, Kanaki N, Rikitake M, Tou M, Murata D, Yanagita E, Kano A, Mitani S, Nomura K, Kitagawa H. |
| タイトル | Chondroitin 4-O-Sulfotransferase Is Indispensable for Sulfation of Chondroitin and Plays an Important Role in Maintaining Normal Life Span and Oxidative Stress Responses in Nematodes. |
| ジャーナル | J Biol Chem |
| Abstract |
Chondroitin sulfate (CS)/chondroitin (Chn) chains are indispensable for embryonic cell division and cytokinesis in the early developmental stages in Caenorhabditis elegans and mice, whereas heparan sulfate (HS) is essential for axon guidance during nervous system development. These data indicate that the fundamental functions of CS and HS are conserved from worms to mammals and that the function of CS/Chn differs from that of HS. Although previous studies have shown that C. elegans produces HS and non-sulfated Chn, whether the organism produces CS remains unclear. Here, we demonstrate that C. elegans produces a small amount of 4-O-sulfated Chn and report the identification of C41C4.1, an orthologue of the human chondroitin 4-O-sulfotransferase gene. Loss of C41C4.1 in C. elegans resulted in a decline in 4-O-sulfation of CS and an increase in the number of sulfated units in HS. C41C4.1 deletion mutants exhibited reduced survival rates after synchronization with sodium hypochlorite. Collectively, these results show for the first time that CS glycans are present in C. elegans and that the Chn 4-O-sulfotransferase responsible for the sulfation plays an important role in protecting nematodes from oxidative stress. |
| 巻・号 | 291(44) |
| ページ | 23294-23304 |
| 公開日 | 2016-10-28 |
| DOI | 10.1074/jbc.M116.757328 |
| PII | S0021-9258(20)35698-2 |
| PMID | 27645998 |
| PMC | PMC5087745 |
| MeSH | Animals Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Division Chondroitin / metabolism* Chondroitin Sulfates / metabolism* Female Heparitin Sulfate / metabolism Male Oxidative Stress* Sequence Deletion Sulfates / metabolism Sulfotransferases / genetics Sulfotransferases / metabolism* |
| IF | 4.106 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm576 |