RRC ID 53561
Author Conway T, Creecy JP, Maddox SM, Grissom JE, Conkle TL, Shadid TM, Teramoto J, San Miguel P, Shimada T, Ishihama A, Mori H, Wanner BL.
Title Unprecedented high-resolution view of bacterial operon architecture revealed by RNA sequencing.
Journal MBio
Abstract 50 genomes). Our data extend recent studies showing unexpected transcriptome complexity in several bacteria and suggest that antisense RNA regulation is widespread. Importance: We precisely mapped the 5' and 3' ends of RNA transcripts across the E. coli K-12 genome by using a single-nucleotide analytical approach. Our resulting high-resolution transcriptome maps show that ca. one-third of E. coli operons are complex, with internal promoters and terminators generating multiple transcription units and allowing differential gene expression within these operons. We discovered extensive antisense transcription that results from more than 500 operons, which fully overlap or extensively overlap adjacent divergent or convergent operons. The genomic regions corresponding to these antisense transcripts are highly conserved in E. coli (including Shigella species), although it remains to be proven whether or not they are functional. Our observations of features unearthed by single-nucleotide transcriptome mapping suggest that deeper layers of transcriptional regulation in bacteria are likely to be revealed in the future.
Volume 5(4)
Pages e01442-14
Published 2014-7-8
DOI 10.1128/mBio.01442-14
PII mBio.01442-14
PMID 25006232
PMC PMC4161252
MeSH Escherichia coli / genetics* Gene Expression Regulation, Bacterial Operon / genetics* Sequence Analysis, RNA / methods* Transcription, Genetic / genetics Transcriptome / genetics
IF 6.689
Resource
Prokaryotes E. coli