| RRC ID |
5357
|
| 著者 |
Iida T, Waki T, Nakamura K, Mukouzaka Y, Kudo T.
|
| タイトル |
The GAF-like-domain-containing transcriptional regulator DfdR is a sensor protein for dibenzofuran and several hydrophobic aromatic compounds.
|
| ジャーナル |
J Bacteriol
|
| Abstract |
Dibenzofuran (DF) is one of the dioxin carbon skeletal compounds used as a model to study the microbial degradation of dioxins. This study analyzed the transcriptional regulation of the DF dioxygenase genes dfdA1 to dfdA4 in the DF-utilizing actinomycetes Rhodococcus sp. strain YK2 and Terrabacter sp. strain YK3. An open reading frame designated dfdR was detected downstream of the dfdC genes. The C-terminal part of the DfdR amino acid sequence has high levels of similarity to several LuxR-type DNA binding helix-turn-helix domains, and a GAF domain sequence in the central part was detected by a domain search analysis. A derivative of YK2 with dfdR disrupted was not able to utilize DF and did not exhibit DF-dependent dfdA1 transcriptional induction ability, and these dysfunctions were compensated for by introduction of dfdR. Promoter analysis of dfdA1 in Rhodococcus strains indicated that activation of the dfdA1 promoter (P(dfdA1)) was dependent on dfdR and DF and not on a metabolite of the DF pathway. The cell extract of a Rhodococcus strain that heterologously expressed DfdR showed electrophoretic mobility shift (EMS) activity for the P(dfdA1) DNA fragment in a DF-dependent manner. In addition, P(dfdA1) activation and EMS activity were observed with hydrophobic aromatic compounds comprising two or more aromatic rings, suggesting that DfdR has broad effector molecule specificity for several hydrophobic aromatic compounds.
|
| 巻・号 |
191(1)
|
| ページ |
123-34
|
| 公開日 |
2009-1-1
|
| DOI |
10.1128/JB.01112-08
|
| PII |
JB.01112-08
|
| PMID |
18952799
|
| PMC |
PMC2612418
|
| MeSH |
Actinomycetales / genetics*
Actinomycetales / metabolism*
Amino Acid Sequence
Benzofurans / metabolism*
Conserved Sequence
DNA, Bacterial / genetics
Dioxygenases / genetics
Dioxygenases / metabolism
Gene Expression Regulation, Bacterial*
Hydrocarbons, Aromatic / metabolism*
Luciferases / genetics
Luciferases / metabolism
Molecular Sequence Data
Multigene Family
Open Reading Frames*
Promoter Regions, Genetic
Rhodococcus / genetics*
Rhodococcus / metabolism*
Sequence Alignment
Sequence Homology, Amino Acid
Trans-Activators / metabolism*
Transcription, Genetic
|
| IF |
3.006
|
| 引用数 |
10
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM2892 |
