RRC ID 53580
Author Melamed S, Naftaly S, Belkin S.
Title Improved detection of antibiotic compounds by bacterial reporter strains achieved by manipulations of membrane permeability and efflux capacity.
Journal Appl Microbiol Biotechnol
Abstract The occurrence of pharmaceuticals, including antibacterial compounds, in the environment has been acknowledged as an emerging and troubling issue in environmental safety; their usage is constantly on the rise, and their effects on the environment are only partially understood. Such compounds can accumulate, contaminate the ecosystem, and contribute to the spreading of antibiotic resistance among bacteria, hindering human health. Bioluminescent Escherichia coli reporter strains, engineered to detect antibiotic compounds by fusing the promoter of the global regulator soxS to the Photorhabdus luminescens luxCDABE cassette, were further modified by altering their membrane permeability and efflux capabilities. This was accomplished by introducing several mutations in the efflux system (ΔemrE, ΔacrB, and ΔtolC) and by overexpressing OmpF, a porin located in the outer membrane that allows passive diffusion of molecules. Combinations of these alterations had a cumulative effect in lowering the detection threshold of several antibiotics, in some of the cases to concentrations reported from pharmaceutical-polluted environments.
Volume 98(5)
Pages 2267-77
Published 2014-3-1
DOI 10.1007/s00253-013-5176-3
PMID 23963273
MeSH Anti-Bacterial Agents / analysis* Anti-Bacterial Agents / pharmacology Bacterial Physiological Phenomena* Biological Transport Biosensing Techniques / methods* Cell Membrane Permeability* Environmental Pollutants / analysis* Environmental Pollutants / pharmacology Escherichia coli / drug effects* Escherichia coli / enzymology Escherichia coli / genetics Gene Deletion Gene Expression Luminescent Proteins / analysis Luminescent Proteins / genetics Photorhabdus / enzymology Photorhabdus / genetics Sensitivity and Specificity
IF 3.53
Times Cited 4
Prokaryotes E. coli