RRC ID 53607
Author Nguyen MH, Ojima Y, Taya M.
Title Enhanced colonization of rpoS-deficient Escherichia coli cells on solid surfaces by reinforced csgA gene expression.
Journal Biocontrol Sci
Abstract Scanning electron microscopy revealed that the rpoS-deficient cells of E. coli K-12 BW25113 (ΔrpoS) increased the number of flagella on the cell surfaces. However, the quantitative analysis of cell colonization showed that the increased number of flagella on ΔrpoS cell surfaces did not cause the enhancement of cell colonization on the surfaces of polyvinyl chloride (PVC), polypropylene (PP) and polystyrene (PS) after 24 h of incubation at 37℃. To facilitate the enhanced expression of curli, the csgA gene was introduced into the ΔrpoS cells. The transformed cells rich in flagella and curli on the cell surfaces were found to make colonies 2-3 times larger than both the wild type and ΔrpoS cells on the PVC, PP and PS surfaces at 37℃. It was thus verified that the reinforcement of csgA gene in the ΔrpoS cells induced the enhanced colonization on the solid surfaces with the increased flagellum and curli expressions.
Volume 19(3)
Pages 147-50
Published 2014
DOI 10.4265/bio.19.147
PII DN/JST.JSTAGE/bio/19.147
PMID 25252647
MeSH Bacterial Proteins / genetics Bacterial Proteins / metabolism Environmental Microbiology Escherichia coli K12 / genetics Escherichia coli K12 / growth & development* Escherichia coli Proteins / biosynthesis* Escherichia coli Proteins / genetics Flagella / metabolism Flagella / ultrastructure Gene Expression* Microscopy, Electron, Scanning Plastics Sigma Factor / deficiency* Sigma Factor / genetics Temperature Time Factors
IF 0.806
Times Cited 0
Prokaryotes E. coli