Reference - Detail
|Author||Sawa N, Zendo T, Kiyofuji J, Fujita K, Himeno K, Nakayama J, Sonomoto K.|
|Title||Identification and characterization of lactocyclicin Q, a novel cyclic bacteriocin produced by Lactococcus sp. strain QU 12.|
|Journal||Appl. Environ. Microbiol.|
Lactococcus sp. strain QU 12, which was isolated from cheese, produced a novel cyclic bacteriocin termed lactocyclicin Q. By using cation-exchange chromatography, hydrophobic interaction chromatography, and reverse-phase high-performance liquid chromatography, lactocyclicin Q was purified from culture supernatant, and its molecular mass was determined to be 6,062.8 Da by mass spectrometry. Lactocyclicin Q has been characterized by its unique antimicrobial spectrum, high level of protease resistance, and heat stability compared to other reported bacteriocins of lactic acid bacteria. The amino acid sequence of lactocyclicin Q was determined chemically, and this compound is composed of 61 amino acid residues that have a cyclic structure with linkage between the N and C termini by a peptide bond. It showed no homology to any other antimicrobial peptide, including cyclic bacteriocins. On the basis of the amino acid sequences obtained, the sequence of the gene encoding the prepeptide lactocyclicin Q was obtained. This is the first report of a cyclic bacteriocin purified from a strain belonging to the genus Lactococcus.
|MeSH||Amino Acid Sequence Bacteria / drug effects* Bacteriocins / chemistry Bacteriocins / genetics Bacteriocins / isolation & purification* Bacteriocins / pharmacology* Base Sequence Chromatography, Ion Exchange DNA, Bacterial / chemistry DNA, Bacterial / genetics Hot Temperature Lactococcus / metabolism* Mass Spectrometry Molecular Sequence Data Molecular Weight Peptide Hydrolases / metabolism Sequence Analysis, DNA Sequence Homology, Amino Acid|
|WOS Category||BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY|
|General Microbes||JCM2257 JCM7638 JCM5706 JCM1157 JCM1134 JCM1059 JCM1132 JCM1164 JCM10707 JCM6124 JCM12495 JCM5885 JCM5890 JCM8797 JCM5887 JCM5804 JCM5803 JCM5707 JCM5802 JCM2257 JCM2504 JCM1465 JCM2152|