RRC ID 53625
Author Frohnecke N, Klein S, Seeber F.
Title Protein-protein interaction studies provide evidence for electron transfer from ferredoxin to lipoic acid synthase in Toxoplasma gondii.
Journal FEBS Lett
Abstract The only known redox system in the apicoplast, a plastid-like organelle of apicomplexan parasites, is ferredoxin and ferredoxin-associated reductase. Ferredoxin donates electrons to different enzymes, presumably including lipoate synthase (LipA), which is essential for fatty acid biosynthesis. We recombinantly expressed and characterized LipA from the protozoan parasite Toxoplasma gondii, generated LipA-specific antibodies and confirmed the apicoplast localization of LipA. Electron transfer from ferredoxin to LipA would require direct protein-protein interaction. Such a robust interaction between the two proteins was demonstrated in both yeast and bacterial two-hybrid systems. Taken together, our results provide strong evidence for a role of ferredoxin as an electron donor to LipA.
Volume 589(1)
Pages 31-6
Published 2015-1-2
DOI 10.1016/j.febslet.2014.11.020
PII S0014-5793(14)00825-4
PMID 25433292
MeSH Electron Transport / physiology Ferredoxins / genetics Ferredoxins / metabolism* Protozoan Proteins / genetics Protozoan Proteins / metabolism* Sulfurtransferases / genetics Sulfurtransferases / metabolism* Toxoplasma / genetics Toxoplasma / metabolism*
IF 3.057
Times Cited 3
Prokaryotes E. coli