Reference - Detail
| RRC ID | 53631 |
|---|---|
| Author | Wu H, Tuli L, Bennett GN, San KY. |
| Title | Metabolic transistor strategy for controlling electron transfer chain activity in Escherichia coli. |
| Journal | Metab Eng |
| Abstract |
A novel strategy to finely control a large metabolic flux by using a "metabolic transistor" approach was established. In this approach a small change in the level or availability of an essential component for the process is controlled by adding a competitive reaction that affects a precursor or an intermediate in its biosynthetic pathway. The change of the basal level of the essential component, considered as a base current in a transistor, has a large effect on the flux through the major pathway. In this way, the fine-tuning of a large flux can be accomplished. The "metabolic transistor" strategy was applied to control electron transfer chain function by manipulation of the quinone synthesis pathway in Escherichia coli. The achievement of a theoretical yield of lactate production under aerobic conditions via this strategy upon manipulation of the biosynthetic pathway of the key participant, ubiquinone-8 (Q8), in an E. coli strain provides an in vivo, genetically tunable means to control the activity of the electron transfer chain and manipulate the production of reduced products while limiting consumption of oxygen to a defined amount. |
| Volume | 28 |
| Pages | 159-168 |
| Published | 2015-3-1 |
| DOI | 10.1016/j.ymben.2015.01.002 |
| PII | S1096-7176(15)00004-X |
| PMID | 25596510 |
| PMC | PMC4355220 |
| MeSH | Electron Transport Chain Complex Proteins* / genetics Electron Transport Chain Complex Proteins* / metabolism Escherichia coli* / enzymology Escherichia coli* / genetics Escherichia coli Proteins* / genetics Escherichia coli Proteins* / metabolism Lactic Acid / metabolism Oxygen Consumption / genetics* Ubiquinone / genetics Ubiquinone / metabolism |
| IF | 7.263 |
| Times Cited | 10 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| Prokaryotes E. coli | |