Reference - Detail
| RRC ID | 53636 |
|---|---|
| Author | Ojima Y, Suparman A, Nguyen MH, Sakka M, Sakka K, Taya M. |
| Title | Exopolysaccharide assay in Escherichia coli microcolonies using a cleavable fusion protein of GFP-labeled carbohydrate-binding module. |
| Journal | J Microbiol Methods |
| Abstract |
A fused protein composed of a carbohydrate-binding module and green fluorescence protein (GFP) was developed to measure the exopolysaccharides (EPShs) present in Escherichia coli microcolonies. The cleavage of the GFP part of this protein using a site-specific protease allowed for the non-invasive and quantitative evaluation of the EPShs. |
| Volume | 114 |
| Pages | 75-7 |
| Published | 2015-7-1 |
| DOI | 10.1016/j.mimet.2015.05.013 |
| PII | S0167-7012(15)00152-9 |
| PMID | 25978970 |
| MeSH | Escherichia coli / chemistry* Escherichia coli / growth & development* Genes, Reporter Green Fluorescent Proteins / analysis Green Fluorescent Proteins / genetics Polysaccharides, Bacterial / analysis* Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism |
| IF | 1.701 |
| Times Cited | 2 |
| Resource | |
| Prokaryotes E. coli | |