論文 - 詳細
| RRC ID | 53658 |
|---|---|
| 著者 | Aluri S, Rex K, Varshney U. |
| タイトル | Simultaneous presence of fhs and purT genes is disadvantageous for the fitness of Escherichia coli growth. |
| ジャーナル | FEMS Microbiol Lett |
| Abstract |
In bacteria, alternate mechanisms are known to synthesize N(10)-formyltetrahydrofolate (N(10)-formyl-THF) and formyl glycinamide ribotide (fGAR), which are important in purine biosynthesis. In one of the mechanisms, a direct transfer of one carbon unit from formate allows Fhs to convert tetrahydrofolate to N(10)-formyl-THF, and PurT to convert glycinamide ribotide (GAR) to fGAR. Our bioinformatics analysis of fhs and purT genes (encoding Fhs and PurT) showed that in a majority of bacteria (∼94%), their presence was mutually exclusive. A large number of organisms possessing fhs lacked purT and vice versa. The phenomenon is so penetrating that even within a genus (Bacillus) if a species possessed fhs it lacked purT and vice versa. To investigate physiological importance of this phenomenon, we used Escherichia coli, which naturally lacks fhs (and possesses purT) as model. We generated strains, which possessed fhs and purT genes in singles or together. Deletion of purT from E. coli in the presence or absence of fhs did not confer a detectable growth disadvantage in pure cultures. However, growth competition assays revealed that the strains possessing either of the single genes outcompeted those possessing both the genes suggesting that mutual exclusion of purT and fhs in organisms confers fitness advantage in mixed cultures. |
| 巻・号 | 362(14) |
| 公開日 | 2015-7-1 |
| DOI | 10.1093/femsle/fnv101 |
| PII | fnv101 |
| PMID | 26100103 |
| MeSH | Amino Acid Sequence Cloning, Molecular Computational Biology Escherichia coli / genetics* Escherichia coli / growth & development* Escherichia coli Proteins / genetics* Formate-Tetrahydrofolate Ligase / genetics* Gene Deletion Genetic Fitness* Glycine / analogs & derivatives Glycine / metabolism Hydroxymethyl and Formyl Transferases / genetics* Ribonucleotides / metabolism |
| IF | 1.987 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | |