RRC ID 53658
Author Aluri S, Rex K, Varshney U.
Title Simultaneous presence of fhs and purT genes is disadvantageous for the fitness of Escherichia coli growth.
Journal FEMS Microbiol Lett
Abstract In bacteria, alternate mechanisms are known to synthesize N(10)-formyltetrahydrofolate (N(10)-formyl-THF) and formyl glycinamide ribotide (fGAR), which are important in purine biosynthesis. In one of the mechanisms, a direct transfer of one carbon unit from formate allows Fhs to convert tetrahydrofolate to N(10)-formyl-THF, and PurT to convert glycinamide ribotide (GAR) to fGAR. Our bioinformatics analysis of fhs and purT genes (encoding Fhs and PurT) showed that in a majority of bacteria (∼94%), their presence was mutually exclusive. A large number of organisms possessing fhs lacked purT and vice versa. The phenomenon is so penetrating that even within a genus (Bacillus) if a species possessed fhs it lacked purT and vice versa. To investigate physiological importance of this phenomenon, we used Escherichia coli, which naturally lacks fhs (and possesses purT) as model. We generated strains, which possessed fhs and purT genes in singles or together. Deletion of purT from E. coli in the presence or absence of fhs did not confer a detectable growth disadvantage in pure cultures. However, growth competition assays revealed that the strains possessing either of the single genes outcompeted those possessing both the genes suggesting that mutual exclusion of purT and fhs in organisms confers fitness advantage in mixed cultures.
Volume 362(14)
Published 2015-7-1
DOI 10.1093/femsle/fnv101
PII fnv101
PMID 26100103
MeSH Amino Acid Sequence Cloning, Molecular Computational Biology Escherichia coli / genetics* Escherichia coli / growth & development* Escherichia coli Proteins / genetics* Formate-Tetrahydrofolate Ligase / genetics* Gene Deletion Genetic Fitness* Glycine / analogs & derivatives Glycine / metabolism Hydroxymethyl and Formyl Transferases / genetics* Ribonucleotides / metabolism
IF 1.987
Times Cited 1
Prokaryotes E. coli