| RRC ID |
5366
|
| 著者 |
Doi K, Fujino Y, Inagaki F, Kawatsu R, Tahara M, Ohshima T, Okaue Y, Yokoyama T, Iwai S, Ogata S.
|
| タイトル |
Stimulation of expression of a silica-induced protein (Sip) in Thermus thermophilus by supersaturated silicic acid.
|
| ジャーナル |
Appl Environ Microbiol
|
| Abstract |
The effects of silicic acid on the growth of Thermus thermophilus TMY, an extreme thermophile isolated from a siliceous deposit formed from geothermal water at a geothermal power plant in Japan, were examined at 75 degrees C. At concentrations higher than the solubility of amorphous silica (400 to 700 ppm SiO(2)), a silica-induced protein (Sip) was isolated from the cell envelope fraction of log-phase TMY cells grown in the presence of supersaturated silicic acid. Two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed the molecular mass and pI of Sip to be about 35 kDa and 9.5, respectively. Induction of Sip expression occurred within 1 h after the addition of a supersaturating concentration of silicic acid to TM broth. Expression of Sip-like proteins was also observed in other thermophiles, including T. thermophilus HB8 and Thermus aquaticus YT-1. The amino acid sequence of Sip was similar to that of the predicted solute-binding protein of the Fe(3+) ABC transporter in T. thermophilus HB8 (locus tag, TTHA1628; GenBank accession no. NC_006461; GeneID, 3169376). The sip gene (987-bp) product showed 87% identity with the TTHA1628 product and the presumed Fe(3+)-binding protein of T. thermophilus HB27 (locus tag TTC1264; GenBank accession no. NC_005835; GeneID, 2774619). Within the genome, sip is situated as a component of the Fbp-type ABC transporter operon, which contains a palindromic structure immediately downstream of sip. This structure is conserved in other T. thermophilus genomes and may function as a terminator that causes definitive Sip expression in response to silica stress.
|
| 巻・号 |
75(8)
|
| ページ |
2406-13
|
| 公開日 |
2009-4-1
|
| DOI |
10.1128/AEM.02387-08
|
| PII |
AEM.02387-08
|
| PMID |
19233950
|
| PMC |
PMC2675196
|
| MeSH |
Amino Acid Sequence
Bacterial Proteins / biosynthesis*
Bacterial Proteins / chemistry
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
Electrophoresis, Gel, Two-Dimensional
Geologic Sediments / microbiology*
Hot Springs
Japan
Molecular Sequence Data
Molecular Weight
Phylogeny
Proteome / analysis
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Silicic Acid / metabolism*
Thermus thermophilus / drug effects*
Thermus thermophilus / growth & development
Thermus thermophilus / isolation & purification
Thermus thermophilus / metabolism
|
| IF |
4.016
|
| 引用数 |
11
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM10668 |
