論文 - 詳細
| RRC ID | 53669 |
|---|---|
| 著者 | Soma Y, Hanai T. |
| タイトル | Self-induced metabolic state switching by a tunable cell density sensor for microbial isopropanol production. |
| ジャーナル | Metab Eng |
| Abstract |
Chemicals production by engineered microorganisms often requires induction of target gene expression at an appropriate cell density to reduce conflict with cell growth. The lux system in Vibrio fischeri is a well-characterized model for cell density-dependent regulation of gene expression termed quorum sensing (QS). However, there are currently no reports for application of the lux system to microbial chemical production. Here, we constructed a synthetic lux system as a tunable cell density sensor-regulator using a synthetic lux promoter and a positive feedback loop in Escherichia coli. In this system, self-induction of a target gene expression is driven by QS-signal, and its threshold cell density can be changed depending on the concentration of a chemical inducer. We demonstrate auto-redirection of metabolic flux from central metabolic pathways toward a synthetic isopropanol pathway at a desired cell density resulting in a significant increase in isopropanol production. |
| 巻・号 | 30 |
| ページ | 7-15 |
| 公開日 | 2015-7-1 |
| DOI | 10.1016/j.ymben.2015.04.005 |
| PII | S1096-7176(15)00049-X |
| PMID | 25908185 |
| MeSH | 2-Propanol / metabolism* Aliivibrio fischeri / genetics Aliivibrio fischeri / metabolism Bioreactors / microbiology* Escherichia coli / genetics* Escherichia coli / growth & development* Gene Expression Regulation, Bacterial* |
| IF | 7.263 |
| 引用数 | 50 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | |