Reference - Detail
| RRC ID | 53684 |
|---|---|
| Author | Yosef I, Manor M, Kiro R, Qimron U. |
| Title | Temperate and lytic bacteriophages programmed to sensitize and kill antibiotic-resistant bacteria. |
| Journal | Proc Natl Acad Sci U S A |
| Abstract |
The increasing threat of pathogen resistance to antibiotics requires the development of novel antimicrobial strategies. Here we present a proof of concept for a genetic strategy that aims to sensitize bacteria to antibiotics and selectively kill antibiotic-resistant bacteria. We use temperate phages to deliver a functional clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas) system into the genome of antibiotic-resistant bacteria. The delivered CRISPR-Cas system destroys both antibiotic resistance-conferring plasmids and genetically modified lytic phages. This linkage between antibiotic sensitization and protection from lytic phages is a key feature of the strategy. It allows programming of lytic phages to kill only antibiotic-resistant bacteria while protecting antibiotic-sensitized bacteria. Phages designed according to this strategy may be used on hospital surfaces and hand sanitizers to facilitate replacement of antibiotic-resistant pathogens with sensitive ones. |
| Volume | 112(23) |
| Pages | 7267-72 |
| Published | 2015-6-9 |
| DOI | 10.1073/pnas.1500107112 |
| PII | 1500107112 |
| PMID | 26060300 |
| PMC | PMC4466736 |
| MeSH | Bacteria / genetics Bacteria / virology* Bacteriophages / physiology* Clustered Regularly Interspaced Short Palindromic Repeats Drug Resistance, Bacterial* Lysogeny Plasmids |
| IF | 9.412 |
| Times Cited | 154 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 183 |
| Altmetric score changes over past 6months | 6.0 |
| Resource | |
| Prokaryotes E. coli | |