| RRC ID |
53684
|
| Author |
Yosef I, Manor M, Kiro R, Qimron U.
|
| Title |
Temperate and lytic bacteriophages programmed to sensitize and kill antibiotic-resistant bacteria.
|
| Journal |
Proc Natl Acad Sci U S A
|
| Abstract |
The increasing threat of pathogen resistance to antibiotics requires the development of novel antimicrobial strategies. Here we present a proof of concept for a genetic strategy that aims to sensitize bacteria to antibiotics and selectively kill antibiotic-resistant bacteria. We use temperate phages to deliver a functional clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas) system into the genome of antibiotic-resistant bacteria. The delivered CRISPR-Cas system destroys both antibiotic resistance-conferring plasmids and genetically modified lytic phages. This linkage between antibiotic sensitization and protection from lytic phages is a key feature of the strategy. It allows programming of lytic phages to kill only antibiotic-resistant bacteria while protecting antibiotic-sensitized bacteria. Phages designed according to this strategy may be used on hospital surfaces and hand sanitizers to facilitate replacement of antibiotic-resistant pathogens with sensitive ones.
|
| Volume |
112(23)
|
| Pages |
7267-72
|
| Published |
2015-6-9
|
| DOI |
10.1073/pnas.1500107112
|
| PII |
1500107112
|
| PMID |
26060300
|
| PMC |
PMC4466736
|
| MeSH |
Bacteria / genetics
Bacteria / virology*
Bacteriophages / physiology*
Clustered Regularly Interspaced Short Palindromic Repeats
Drug Resistance, Bacterial*
Lysogeny
Plasmids
|
| IF |
9.412
|
| Times Cited |
154
|
| Resource |
| Prokaryotes E. coli |
|
