Reference - Detail
| RRC ID | 53691 |
|---|---|
| Author | Verstraeten N, Knapen WJ, Kint CI, Liebens V, Van den Bergh B, Dewachter L, Michiels JE, Fu Q, David CC, Fierro AC, Marchal K, Beirlant J, Versées W, Hofkens J, Jansen M, Fauvart M, Michiels J. |
| Title | Obg and Membrane Depolarization Are Part of a Microbial Bet-Hedging Strategy that Leads to Antibiotic Tolerance. |
| Journal | Mol Cell |
| Abstract |
Within bacterial populations, a small fraction of persister cells is transiently capable of surviving exposure to lethal doses of antibiotics. As a bet-hedging strategy, persistence levels are determined both by stochastic induction and by environmental stimuli called responsive diversification. Little is known about the mechanisms that link the low frequency of persisters to environmental signals. Our results support a central role for the conserved GTPase Obg in determining persistence in Escherichia coli in response to nutrient starvation. Obg-mediated persistence requires the stringent response alarmone (p)ppGpp and proceeds through transcriptional control of the hokB-sokB type I toxin-antitoxin module. In individual cells, increased Obg levels induce HokB expression, which in turn results in a collapse of the membrane potential, leading to dormancy. Obg also controls persistence in Pseudomonas aeruginosa and thus constitutes a conserved regulator of antibiotic tolerance. Combined, our findings signify an important step toward unraveling shared genetic mechanisms underlying persistence. |
| Volume | 59(1) |
| Pages | 9-21 |
| Published | 2015-7-2 |
| DOI | 10.1016/j.molcel.2015.05.011 |
| PII | S1097-2765(15)00347-0 |
| PMID | 26051177 |
| MeSH | Anti-Bacterial Agents / pharmacology Bacterial Proteins / genetics* Bacterial Toxins / biosynthesis* Bacterial Toxins / genetics Cell Membrane / physiology Drug Resistance, Bacterial / physiology* Escherichia coli / genetics* Escherichia coli Proteins / biosynthesis* Escherichia coli Proteins / genetics GTP-Binding Proteins / genetics* Membrane Potentials / genetics Microbial Sensitivity Tests Protein Structure, Tertiary / genetics Pseudomonas aeruginosa / genetics* |
| IF | 15.584 |
| Times Cited | 119 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 39 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | |