論文 - 詳細
| RRC ID | 53702 |
|---|---|
| 著者 | Adolfsen KJ, Brynildsen MP. |
| タイトル | Futile cycling increases sensitivity toward oxidative stress in Escherichia coli. |
| ジャーナル | Metab Eng |
| Abstract |
Reactive oxygen species (ROS) are toxic molecules utilized by the immune system to combat invading pathogens. Recent evidence suggests that inefficiencies in ATP production or usage can lead to increased endogenous ROS production and sensitivity to oxidative stress in bacteria. With this as inspiration, and knowledge that ATP is required for a number of DNA repair mechanisms, we hypothesized that futile cycling would be an effective way to increase sensitivity to oxidative stress. We developed a mixed integer linear optimization framework to identify experimentally-tractable futile cycles, and confirmed metabolic modeling predictions that futile cycling depresses growth rate, and increases both O2 consumption and ROS production per biomass generated. Further, intracellular ATP was decreased and sensitivity to oxidative stress increased in all actively cycling strains compared to their catalytically inactive controls. This research establishes a fundamental connection between ATP metabolism, endogenous ROS production, and tolerance toward oxidative stress in bacteria. |
| 巻・号 | 29 |
| ページ | 26-35 |
| 公開日 | 2015-5-1 |
| DOI | 10.1016/j.ymben.2015.02.006 |
| PII | S1096-7176(15)00025-7 |
| PMID | 25732623 |
| PMC | PMC4430396 |
| MeSH | Adenosine Triphosphate / genetics Adenosine Triphosphate / metabolism DNA Repair / physiology Escherichia coli K12 / genetics Escherichia coli K12 / metabolism* Oxidative Stress / physiology* Reactive Oxygen Species / metabolism* Substrate Cycling / physiology* |
| IF | 7.263 |
| 引用数 | 30 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | |