| RRC ID |
53709
|
| Author |
Sandoval JM, Arenas FA, García JA, Díaz-Vásquez WA, Valdivia-González M, Sabotier M, Vásquez CC.
|
| Title |
Escherichia coli 6-phosphogluconate dehydrogenase aids in tellurite resistance by reducing the toxicant in a NADPH-dependent manner.
|
| Journal |
Microbiol Res
|
| Abstract |
Exposure to the tellurium oxyanion tellurite (TeO3(2-)) results in the establishment of an oxidative stress status in most microorganisms. Usually, bacteria growing in the presence of the toxicant turn black because of the reduction of tellurite (Te(4+)) to the less-toxic elemental tellurium (Te(0)). In vitro, at least part of tellurite reduction occurs enzymatically in a nicotinamide dinucleotide-dependent reaction. In this work, we show that TeO3(2-) reduction by crude extracts of Escherichia coli overexpressing the zwf gene (encoding glucose-6-phosphate dehydrogenase) takes place preferentially in the presence of NADPH instead of NADH. The enzyme responsible for toxicant reduction was identified as 6-phosphogluconate dehydrogenase (Gnd). The gnd gene showed a subtle induction at short times after toxicant exposure while strains lacking gnd were more susceptible to the toxicant. These results suggest that both NADPH-generating enzymes from the pentose phosphate shunt may be involved in tellurite detoxification and resistance in E. coli.
|
| Volume |
177
|
| Pages |
22-7
|
| Published |
2015-8-1
|
| DOI |
10.1016/j.micres.2015.05.002
|
| PII |
S0944-5013(15)00089-0
|
| PMID |
26211962
|
| MeSH |
Drug Resistance, Bacterial*
Escherichia coli / drug effects
Escherichia coli / enzymology*
Escherichia coli / metabolism*
Inactivation, Metabolic
NADP / metabolism*
Oxidation-Reduction
Phosphogluconate Dehydrogenase / metabolism*
Tellurium / metabolism*
Tellurium / toxicity
|
| IF |
3.97
|
| Times Cited |
2
|
| Resource |
| Prokaryotes E. coli |
|
