RRC ID 53718
Author Kadoya R, Kodama Y, Matsumoto K, Taguchi S.
Title Indirect positive effects of a sigma factor RpoN deletion on the lactate-based polymer production in Escherichia coli.
Journal Bioengineered
Abstract The production of bacterial polyesters, polyhydroxyalkanoates (PHAs), has been improved by several rational approaches such as overexpression and/or engineering of the enzymes directly related to PHA biosynthetic pathways. In this study, a new approach at transcription level has been applied to a new category of the copolymer of lactate (LA) and 3-hydroxybutyrate (3HB), P(LA-co-3HB). When the 4 disrupting mutants of sigma factors in Escherichia coli, rpoN, rpoS, fliA, fecI, were used as platforms for production of P(LA-co-3HB), increases in the production level and LA fraction of the copolymer were observed for the mutant strain with rpoN disruption. These positive impacts on the polymer production were caused in an "indirect manner" via changes in the multiple genes governed by RpoN. A genome-wide engineering by sigma factors would be a versatile approach for the production of value-added products of interest and available for combination with the other beneficial tools.
Volume 6(5)
Pages 307-11
Published 2015-1-1
DOI 10.1080/21655979.2015.1069449
PMID 26218242
PMC PMC4825821
MeSH 3-Hydroxybutyric Acid / isolation & purification 3-Hydroxybutyric Acid / metabolism* Escherichia coli / genetics Escherichia coli / metabolism* Genetic Enhancement / methods* Lactic Acid / isolation & purification Lactic Acid / metabolism* Polyesters Polyhydroxyalkanoates / isolation & purification Polyhydroxyalkanoates / metabolism* Polymers / isolation & purification Polymers / metabolism* Sigma Factor / genetics Sigma Factor / metabolism*
IF 1.639
Times Cited 3
Prokaryotes E. coli