論文 - 詳細
| RRC ID | 53722 |
|---|---|
| 著者 | Takayama Y, Yonekura K. |
| タイトル | Cryogenic coherent X-ray diffraction imaging of biological samples at SACLA: a correlative approach with cryo-electron and light microscopy. |
| ジャーナル | Acta Crystallogr A Found Adv |
| Abstract |
Coherent X-ray diffraction imaging at cryogenic temperature (cryo-CXDI) allows the analysis of internal structures of unstained, non-crystalline, whole biological samples in micrometre to sub-micrometre dimensions. Targets include cells and cell organelles. This approach involves preparing frozen-hydrated samples under controlled humidity, transferring the samples to a cryo-stage inside a vacuum chamber of a diffractometer, and then exposing the samples to coherent X-rays. Since 2012, cryo-coherent diffraction imaging (CDI) experiments have been carried out with the X-ray free-electron laser (XFEL) at the SPring-8 Ångstrom Compact free-electron LAser (SACLA) facility in Japan. Complementary use of cryo-electron microscopy and/or light microscopy is highly beneficial for both pre-checking samples and studying the integrity or nature of the sample. This article reports the authors' experience in cryo-XFEL-CDI of biological cells and organelles at SACLA, and describes an attempt towards reliable and higher-resolution reconstructions, including signal enhancement with strong scatterers and Patterson-search phasing. |
| 巻・号 | 72(Pt 2) |
| ページ | 179-89 |
| 公開日 | 2016-3-1 |
| DOI | 10.1107/S2053273315023980 |
| PII | S2053273315023980 |
| PMID | 26919369 |
| MeSH | Cryoelectron Microscopy / methods* Diagnostic Imaging / methods* Electrons Freezing X-Ray Diffraction / methods* |
| IF | 7.93 |
| 引用数 | 7 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 原核生物(大腸菌) | |