| RRC ID |
53724
|
| 著者 |
Ideguchi Y, Oshikoshi Y, Ryo M, Motoki S, Kuwano T, Tezuka T, Aoki S.
|
| タイトル |
Posttranslationally caused bioluminescence burst of the Escherichia coli luciferase reporter strain.
|
| ジャーナル |
Arch Microbiol
|
| Abstract |
We continuously monitored bioluminescence from a wild-type reporter strain of Escherichia coli (lacp::luc+/WT), which carries the promoter of the lac operon (lacp) fused with the firefly luciferase gene (luc+). This strain showed a bioluminescence burst when shifted into the stationary growth phase. Bioluminescence profiles of other wild-type reporter strains (rpsPp::luc+ and argAp::luc+) and gene-deletion reporter strains (lacp::luc+/crp- and lacp::luc+/lacI-) indicate that transcriptional regulation is not responsible for generation of the burst. Consistently, changes in the luciferase protein levels did not recapitulate the profile of the burst. On the other hand, dissolved oxygen levels increased over the period across the burst, suggesting that the burst is, at least partially, caused by an increase in intracellular oxygen levels. We discuss limits of the firefly luciferase when used as a reporter for gene expression and its potential utility for monitoring metabolic changes in cells.
|
| 巻・号 |
198(1)
|
| ページ |
35-41
|
| 公開日 |
2016-1-1
|
| DOI |
10.1007/s00203-015-1165-5
|
| PII |
10.1007/s00203-015-1165-5
|
| PMID |
26506945
|
| MeSH |
Escherichia coli / enzymology*
Escherichia coli / genetics*
Gene Expression Regulation, Bacterial
Genes, Reporter / genetics
Luciferases / genetics
Luciferases / metabolism*
Oxygen / metabolism
Promoter Regions, Genetic / genetics
|
| IF |
1.884
|
| 引用数 |
0
|
| リソース情報 |
| 原核生物(大腸菌) |
|
