RRC ID 53724
Author Ideguchi Y, Oshikoshi Y, Ryo M, Motoki S, Kuwano T, Tezuka T, Aoki S.
Title Posttranslationally caused bioluminescence burst of the Escherichia coli luciferase reporter strain.
Journal Arch Microbiol
Abstract We continuously monitored bioluminescence from a wild-type reporter strain of Escherichia coli (lacp::luc+/WT), which carries the promoter of the lac operon (lacp) fused with the firefly luciferase gene (luc+). This strain showed a bioluminescence burst when shifted into the stationary growth phase. Bioluminescence profiles of other wild-type reporter strains (rpsPp::luc+ and argAp::luc+) and gene-deletion reporter strains (lacp::luc+/crp- and lacp::luc+/lacI-) indicate that transcriptional regulation is not responsible for generation of the burst. Consistently, changes in the luciferase protein levels did not recapitulate the profile of the burst. On the other hand, dissolved oxygen levels increased over the period across the burst, suggesting that the burst is, at least partially, caused by an increase in intracellular oxygen levels. We discuss limits of the firefly luciferase when used as a reporter for gene expression and its potential utility for monitoring metabolic changes in cells.
Volume 198(1)
Pages 35-41
Published 2016-1-1
DOI 10.1007/s00203-015-1165-5
PII 10.1007/s00203-015-1165-5
PMID 26506945
MeSH Escherichia coli / enzymology* Escherichia coli / genetics* Gene Expression Regulation, Bacterial Genes, Reporter / genetics Luciferases / genetics Luciferases / metabolism* Oxygen / metabolism Promoter Regions, Genetic / genetics
IF 1.884
Times Cited 0
Prokaryotes E. coli