Reference - Detail
| RRC ID | 53742 |
|---|---|
| Author | Nomura Y, Ohno S, Nishikawa K, Yokogawa T. |
| Title | Correlation between the stability of tRNA tertiary structure and the catalytic efficiency of a tRNA-modifying enzyme, archaeal tRNA-guanine transglycosylase. |
| Journal | Genes Cells |
| Abstract |
In many archaeal tRNAs, archaeosine is found at position 15. During archaeosine biosynthesis, archaeal tRNA-guanine transglycosylase (ArcTGT) first replaces the guanine base at position 15 with 7-cyano-7-deazaguanine (preQ0). In this study, we investigated whether modified nucleosides in tRNA substrates would affect ArcTGT incorporation of preQ0. We prepared a series of hypomodified tRNAs(Ser)(GGA) from Escherichia coli strains lacking each tRNA-modifying enzyme. Measurement of ArcTGT kinetic parameters with the various tRNAs(Ser)(GGA) as substrates showed that the Km decreased due to the lack of modified nucleosides. The tRNAs(Ser)(GGA) melting profiles resulted in experimental evidence showing that each modified nucleoside in tRNA(Ser)(GGA) enhanced tRNA stability. Furthermore, the ArcTGT K(m) strongly correlated with the melting temperature (T(m)), suggesting that the unstable tRNA containing fewer modified nucleosides served as a better ArcTGT substrate. These results show that preQ0 incorporation into tRNA by ArcTGT takes place early in the archaeal tRNA modification process. |
| Volume | 21(1) |
| Pages | 41-52 |
| Published | 2016-1-1 |
| DOI | 10.1111/gtc.12317 |
| PMID | 26663416 |
| MeSH | Archaea / enzymology Archaea / genetics Base Sequence Biocatalysis* Escherichia coli / metabolism Guanine / metabolism Kinetics Methanosarcina / enzymology* Molecular Sequence Data Nucleic Acid Conformation* Pentosyltransferases / metabolism* RNA Stability / genetics* RNA, Messenger / genetics RNA, Messenger / metabolism RNA, Transfer / chemistry* RNA, Transfer / genetics RNA, Transfer / metabolism* Substrate Specificity Transition Temperature |
| IF | 1.655 |
| Times Cited | 7 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | News |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | |