Reference - Detail
| RRC ID | 53743 |
|---|---|
| Author | Schmidt A, Kochanowski K, Vedelaar S, Ahrné E, Volkmer B, Callipo L, Knoops K, Bauer M, Aebersold R, Heinemann M. |
| Title | The quantitative and condition-dependent Escherichia coli proteome. |
| Journal | Nat Biotechnol |
| Abstract |
Measuring precise concentrations of proteins can provide insights into biological processes. Here we use efficient protein extraction and sample fractionation, as well as state-of-the-art quantitative mass spectrometry techniques to generate a comprehensive, condition-dependent protein-abundance map for Escherichia coli. We measure cellular protein concentrations for 55% of predicted E. coli genes (>2,300 proteins) under 22 different experimental conditions and identify methylation and N-terminal protein acetylations previously not known to be prevalent in bacteria. We uncover system-wide proteome allocation, expression regulation and post-translational adaptations. These data provide a valuable resource for the systems biology and broader E. coli research communities. |
| Volume | 34(1) |
| Pages | 104-10 |
| Published | 2016-1-1 |
| DOI | 10.1038/nbt.3418 |
| PII | nbt.3418 |
| PMID | 26641532 |
| PMC | PMC4888949 |
| MeSH | Escherichia coli / metabolism* Escherichia coli Proteins / metabolism* Mass Spectrometry Proteome* |
| IF | 36.553 |
| Times Cited | 228 |
| Resource | |
| Prokaryotes E. coli | |