RRC ID |
53762
|
Author |
Bolt EL, Jenkins T, Russo VM, Ahmed S, Cavey J, Cass SD.
|
Title |
Identification of Escherichia coli ygaQ and rpmG as novel mitomycin C resistance factors implicated in DNA repair.
|
Journal |
Biosci Rep
|
Abstract |
Using the ASKA (A Complete Set of Escherichia coli K-12 ORF Archive) library for genome-wide screening of E. coli proteins we identified that expression of ygaQ and rpmG promotes mitomycin C resistance (MMC(R)). YgaQ mediated MMC(R) was independent of homologous recombination involving RecA or RuvABC, but required UvrD. YgaQ is an uncharacterized protein homologous with α-amylases that we identified to have nuclease activity directed to ssDNA of 5' flaps. Nuclease activity was inactivated by mutation of two amino acid motifs, which also abolished MMC(R). RpmG is frequently annotated as a bacterial ribosomal protein, although forms an operon with MutM glycosylase and a putative deubiquitinating (DUB) enzyme, YicR. RpmG associated MMC(R) was dependent on MutM. MMC(R) from RpmG resembles DNA repair phenotypes reported for 'idiosyncratic ribosomal proteins' in eukaryotes.
|
Volume |
36(1)
|
Pages |
e00290
|
Published |
2015-12-24
|
DOI |
10.1042/BSR20150249
|
PII |
BSR20150249
|
PMID |
26704888
|
PMC |
PMC4725251
|
MeSH |
Drug Resistance, Bacterial / drug effects*
Drug Resistance, Bacterial / physiology
Escherichia coli K12 / genetics
Escherichia coli K12 / metabolism*
Escherichia coli Proteins / biosynthesis*
Escherichia coli Proteins / genetics
Gene Expression Regulation, Bacterial / drug effects*
Gene Expression Regulation, Bacterial / genetics
Mitomycin / pharmacology*
Ribosomal Proteins / biosynthesis*
Ribosomal Proteins / genetics
|
IF |
2.942
|
Times Cited |
1
|
Resource |
Prokaryotes E. coli |
|