RRC ID 53762
著者 Bolt EL, Jenkins T, Russo VM, Ahmed S, Cavey J, Cass SD.
タイトル Identification of Escherichia coli ygaQ and rpmG as novel mitomycin C resistance factors implicated in DNA repair.
ジャーナル Biosci Rep
Abstract Using the ASKA (A Complete Set of Escherichia coli K-12 ORF Archive) library for genome-wide screening of E. coli proteins we identified that expression of ygaQ and rpmG promotes mitomycin C resistance (MMC(R)). YgaQ mediated MMC(R) was independent of homologous recombination involving RecA or RuvABC, but required UvrD. YgaQ is an uncharacterized protein homologous with α-amylases that we identified to have nuclease activity directed to ssDNA of 5' flaps. Nuclease activity was inactivated by mutation of two amino acid motifs, which also abolished MMC(R). RpmG is frequently annotated as a bacterial ribosomal protein, although forms an operon with MutM glycosylase and a putative deubiquitinating (DUB) enzyme, YicR. RpmG associated MMC(R) was dependent on MutM. MMC(R) from RpmG resembles DNA repair phenotypes reported for 'idiosyncratic ribosomal proteins' in eukaryotes.
巻・号 36(1)
ページ e00290
公開日 2015-12-24
DOI 10.1042/BSR20150249
PII BSR20150249
PMID 26704888
PMC PMC4725251
MeSH Drug Resistance, Bacterial / drug effects* Drug Resistance, Bacterial / physiology Escherichia coli K12 / genetics Escherichia coli K12 / metabolism* Escherichia coli Proteins / biosynthesis* Escherichia coli Proteins / genetics Gene Expression Regulation, Bacterial / drug effects* Gene Expression Regulation, Bacterial / genetics Mitomycin / pharmacology* Ribosomal Proteins / biosynthesis* Ribosomal Proteins / genetics
IF 2.942
引用数 1
リソース情報
原核生物(大腸菌)