論文 - 詳細
| RRC ID | 53774 |
|---|---|
| 著者 | Kurokawa M, Seno S, Matsuda H, Ying BW. |
| タイトル | Correlation between genome reduction and bacterial growth. |
| ジャーナル | DNA Res |
| Abstract |
Genome reduction by removing dispensable genomic sequences in bacteria is commonly used in both fundamental and applied studies to determine the minimal genetic requirements for a living system or to develop highly efficient bioreactors. Nevertheless, whether and how the accumulative loss of dispensable genomic sequences disturbs bacterial growth remains unclear. To investigate the relationship between genome reduction and growth, a series of Escherichia coli strains carrying genomes reduced in a stepwise manner were used. Intensive growth analyses revealed that the accumulation of multiple genomic deletions caused decreases in the exponential growth rate and the saturated cell density in a deletion-length-dependent manner as well as gradual changes in the patterns of growth dynamics, regardless of the growth media. Accordingly, a perspective growth model linking genome evolution to genome engineering was proposed. This study provides the first demonstration of a quantitative connection between genomic sequence and bacterial growth, indicating that growth rate is potentially associated with dispensable genomic sequences. |
| 巻・号 | 23(6) |
| ページ | 517-525 |
| 公開日 | 2016-12-1 |
| DOI | 10.1093/dnares/dsw035 |
| PII | dsw035 |
| PMID | 27374613 |
| PMC | PMC5144675 |
| MeSH | Cell Proliferation* Escherichia coli / genetics Escherichia coli / growth & development Genome, Bacterial* Genomic Instability* Models, Genetic* Sequence Deletion |
| IF | 4.009 |
| 引用数 | 15 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | |