| RRC ID |
53781
|
| 著者 |
Matsumoto A, Sekoguchi A, Imai J, Kondo K, Shibata Y, Maeda S.
|
| タイトル |
Natural Escherichia coli strains undergo cell-to-cell plasmid transformation.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Horizontal gene transfer is a strong tool that allows bacteria to adapt to various environments. Although three conventional mechanisms of horizontal gene transfer (transformation, transduction, and conjugation) are well known, new variations of these mechanisms have also been observed. We recently reported that DNase-sensitive cell-to-cell transfer of nonconjugative plasmids occurs between laboratory strains of Escherichia coli in co-culture. We termed this phenomenon "cell-to-cell transformation." In this report, we found that several combinations of Escherichia coli collection of reference (ECOR) strains, which were co-cultured in liquid media, resulted in DNase-sensitive cell-to-cell transfer of antibiotic resistance genes. Plasmid isolation of these new transformants demonstrated cell-to-cell plasmid transfer between the ECOR strains. Natural transformation experiments, using a combination of purified plasmid DNA and the same ECOR strains, revealed that cell-to-cell transformation occurs much more frequently than natural transformation under the same culture conditions. Thus, cell-to-cell transformation is both unique and effective. In conclusion, this study is the first to demonstrate cell-to-cell plasmid transformation in natural E. coli strains.
|
| 巻・号 |
481(1-2)
|
| ページ |
59-62
|
| 公開日 |
2016-12-2
|
| DOI |
10.1016/j.bbrc.2016.11.018
|
| PII |
S0006-291X(16)31873-3
|
| PMID |
27833021
|
| MeSH |
Cell Communication / genetics
DNA, Bacterial / genetics*
Escherichia coli / classification
Escherichia coli / cytology*
Escherichia coli / genetics*
Gene Transfer, Horizontal / genetics*
Plasmids / genetics*
Species Specificity
Transformation, Bacterial / genetics*
|
| IF |
2.985
|
| 引用数 |
6
|
| リソース情報 |
| 原核生物(大腸菌) |
|
